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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Citations
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneGFP.Cre
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Backbone manufacturerJacks Lab
- Backbone size (bp) 9300
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Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer MSCV rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byUbC.Luciferase.PGK.Cre was a kind gift of M. Dupage
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP expression driven by UbC promoter. Cre expression driven by PGK promoter.
For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP.Cre empty vector was a gift from Tyler Jacks (Addgene plasmid # 20781 ; http://n2t.net/addgene:20781 ; RRID:Addgene_20781)
