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pCMV-dR8.2 dvpr
(Plasmid #8455)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8455 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 8455-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    pHR' CMV 8.2 deltaR
  • Alt name
    pCMV-dR8.2 dvpr
  • Alt name
    pCMVdR8.2 dvpr

Cloning Information

Resource Information

Depositor Comments

Packaging plasmid for Lentiviral constructs (vpr is removed). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Information for Cloning Grade DNA (Catalog # 8455-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-dR8.2 dvpr was a gift from Bob Weinberg (Addgene plasmid # 8455 ; http://n2t.net/addgene:8455 ; RRID:Addgene_8455)
  • For your References section:

    Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD. RNA 2003 Apr;9(4):493-501. 10.1261/rna.2192803 PubMed 12649500