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Lentivirus-delivered stable gene silencing by RNAi in primary cells.
Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
RNA 2003 Apr;9(4):493-501.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
8449pUMVCPackaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
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8450Retrohair
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8451Lentihair s1GFP
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8452pMKO.1 puro
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8453pLKO.1 puro3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.
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8454pCMV-VSV-GEnvelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
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8455pCMV-dR8.2 dvpr2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
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