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Torsten Wittmann Lab Plasmids


The Torsten Wittmann Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T J Cell Biol. 2009 Mar 23. 184(6):895-908.
Analysis of microtubule dynamic instability using a plus-end growth marker. Matov A, Applegate K, Kumar P, Thoma C, Krek W, Danuser G, Wittmann T Nat Methods. 2010 Sep . 7(9):761-8.
EB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Gierke S, Wittmann T Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5.
Doublecortin Is Excluded from Growing Microtubule Ends and Recognizes the GDP-Microtubule Lattice. Ettinger A, van Haren J, Ribeiro SA, Wittmann T Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 10.1016/j.cub.2016.04.020. Epub 2016 May 26.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24327pShuttle-EGFP-tubulinTubulin (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pShuttle-CMV TUBA1B K-ALPHA-1 Add to Cart
24371pShuttle-mRFP-gsk3 s9aGSK3beta(S9A) (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pShuttle-CMV GSK3B Add to Cart
24372pShuttle-EGFP-CLASP2 340-1362CLASP2 (340-1362) (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pShuttle-CMV CLASP2 Add to Cart
24373pShuttle-EGFP-CLASP2 340-1362 9xS/ACLASP2 (340-1362) 9xS/A (Homo sapiens)Mammalian ExpressionGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pShuttle-CMV CLASP2 Add to Cart
24383pShuttle-EGFP-CLASP2 340-1084CLASP2 (340-1084) (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pShuttle-CMV CLASP2 Add to Cart
24384pShuttle-EGFP-CLASP2 340-1084 9xS/ACLASP2 (340-1084) 9xS/A (Homo sapiens)Mammalian ExpressionGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pShuttle-CMV CLASP2 Add to Cart
24409pEGFP-CLASP2 512-650CLASP2 (512-650) (Homo sapiens)Mammalian ExpressionGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pEGFP-C1 CLASP2 Add to Cart
24410pEGFP-CLASP2 512-650 9xS/ACLASP2 (512-650) 9xS/A (Homo sapiens)Mammalian ExpressionGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pEGFP-C1 CLASP2 Add to Cart
24411pAdEasy-EGFP-TubulinTubulin (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pAdEasy-1 TUBA1B K-ALPHA-1 Add to Cart
24521pAdEasy-mRFP-gsk3 beta S9AGSK3beta(S9A) (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pAdEasy-1 GSK3B Add to Cart
24522pAdEasy-EGFP-CLASP2 340-1362CLASP2 (340-1362) (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pAdEasy-1 CLASP2 Add to Cart
24523pAdEasy-EGFP-CLASP2 340-1362 9xS/ACLASP2 (340-1362) 9xS/A (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pAdEasy-1 CLASP2 Add to Cart
24524pAdEasy-EGFP-CLASP2 340-1084CLASP2 (340-1084) (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pAdEasy-1 CLASP2 Add to Cart
24525pAdEasy-EGFP-CLASP2 340-1084 9xS/ACLASP2 (340-1084) 9xS/A (Homo sapiens)Mammalian Expression, AdenoviralGSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. J Cell Biol. 2009 Mar 23. 184(6):895-908. pAdEasy-1 CLASP2 Add to Cart
26767pAdEasy mCherry-TubulinTubulin (Homo sapiens)Mammalian Expression, AdenoviralAnalysis of microtubule dynamic instability using a plus-end growth marker. Nat Methods. 2010 Sep . 7(9):761-8. pAdEasy-1 TUBA1B K-ALPHA-1 Add to Cart
26768pShuttle mCherry-TubulinTubulin (Homo sapiens)Mammalian Expression, AdenoviralAnalysis of microtubule dynamic instability using a plus-end growth marker. Nat Methods. 2010 Sep . 7(9):761-8. pShuttle-CMV TUBA1B K-ALPHA-1 Add to Cart
37827pEB1-2xEGFPEB1 (Homo sapiens)Mammalian ExpressionEB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. pEGFP-N1 MAPRE1 EB1 Add to Cart
37828pEB1deltaC-2xEGFPEB1 (Homo sapiens)Mammalian ExpressionEB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. pEGFP-N1
  • Tags / Fusion Proteins
    • EGFP (C terminal on insert)
    • EGFP (C terminal on insert)
  • MAPRE1 EB1
    Add to Cart
    37829pShuttle-mCherry-EB1-CEB1 (Homo sapiens)Mammalian Expression, AdenoviralEB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. pShuttle MAPRE1 EB1 Add to Cart
    37925pEB1deltaC-2xEGFP shRNA resistantEB1 (Homo sapiens)Mammalian ExpressionEB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. pEGFP-N1
  • Tags / Fusion Proteins
    • EGFP (C terminal on insert)
    • EGFP (C terminal on insert)
  • MAPRE1 EB1
    Add to Cart
    37927pLKO.1 EB1 shRNA #3EB1 shRNA #3 (Homo sapiens)Mammalian Expression, Lentiviral, RNAiEB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. pLKO.1 MAPRE1 EB1 Add to Cart
    37928pLKO.1 EB1 shRNA #1EB1 shRNA #1 (Homo sapiens)Mammalian Expression, Lentiviral, RNAiEB1-Recruited Microtubule +TIP Complexes Coordinate Protrusion Dynamics during 3D Epithelial Remodeling. Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5. pLKO.1 MAPRE1 EB1 Add to Cart
    80599pLenti6-DCX-EGFPDoublecortin (Homo sapiens)LentiviralDoublecortin Is Excluded from Growing Microtubule Ends and Recognizes the GDP-Microtubule Lattice. Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 10.1016/j.cub.2016.04.020. Epub 2016 May 26. pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152) pLenti6/V5-DEST DCX DBCN, DC, LISX, SCLH, XLIS Add to Cart
    80603pEB1deltaC-mCherryEB1 (Homo sapiens)Mammalian ExpressionDoublecortin Is Excluded from Growing Microtubule Ends and Recognizes the GDP-Microtubule Lattice. Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 10.1016/j.cub.2016.04.020. Epub 2016 May 26. Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end pmCherry-N1 MAPRE1 EB1 Add to Cart