164,909 results

1. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1 and dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA-Sumo2 WT

   Plasmid

   #48967

   Purpose

   Expresses HA SUMO2 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSGV-1D3-28Z All ITAMs intact

   Plasmid

   #107226

   Purpose

   Anti-mouse CD19 CAR with CD3 ITAMs intact

   Depositor

   Steven Rosenberg, James Kochenderfer

   Article

   Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

   Insert

   1D3-28Z All ITAMs intact
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mutation

   None

   Promoter

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. tFucci(CA)5

   Plasmid

   #153521

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(CA)5.
   

   Use

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIRV-AP-1-mCherry

   Plasmid

   #118095

   Purpose

   Fluorescent reporter for AP-1 activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with AP-1 responsive element

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. M50 Super 8x TOPFlash

   Plasmid

   #12456

   Purpose

   Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-MAPPER

   Plasmid

   #117721

   Purpose

   MAPPER Selectively Labels ER-PM Junctions

   Depositor

   Jen Liou

   Article

   Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

   Insert

   MAPPER
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-S5E2-dTom-nlsdTom

    Plasmid

    #135630

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK444 (EF1-OsTIR1(F74G) Puro)

    Plasmid

    #214374

    Purpose

    EF1-OsTIR1(F74G) (PiggyBac)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    EF1-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mScarletI3-6DG5-MAPPER

    Plasmid

    #222421

    Purpose

    To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    MAPPER
    

    Use

    Tags

    mScarletI3-6DG5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-S5E2-GFP-fGFP

    Plasmid

    #135631

    Purpose

    AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    GFP-fGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-DEST47 PINK1 C-GFP

    Plasmid

    #13316

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPLICS LY-MT Short P2A

    Plasmid

    #213614

    Purpose

    Detect the short-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid

    #203652

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire-IRES-H2B-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. anxA1-GFP

    Plasmid

    #107194

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A1
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV Puro DEST (w118-1)

    Plasmid

    #17452

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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