167,600 results

1. pLV-puro-N-Flag-h-SNRNP40

   Plasmid

   #134254

   Purpose

   Lentivector encoding Flag-tagged human SNRNP40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   SNRP40 (SNRNP40 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-dCasRx-METTL3-PURO

   Plasmid

   #174218

   Purpose

   Expresses dCasRx-METTL3 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-METTL3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   pMSCV

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV5)

   Viral Prep

   #100837-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

4. mCherry-Alpha-Actinin2-N-18

   Plasmid

   #54974

   Purpose

   Localization: Alpha Actinin, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Alpha-Actinin2 (ACTN2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. lenti-sgRNA-BSD

   Plasmid

   #175583

   Purpose

   Expresses sgRNA for CasRx in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9-mCherry-Frame +0

   Plasmid

   #66939

   Purpose

   CRISPaint frame selector +0

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +0
   

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-mCherry (AAV Retrograde)

   Viral Prep

   #114470-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

8. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N2-COL1A1

   Plasmid

   #66602

   Purpose

   Expresses procollagen 1 alpha 1 as a GFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Expression

   Mammalian

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 His6-mEGFP-D4

    Plasmid

    #226372

    Purpose

    Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4 (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209653

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Mutation

    A95A.A119L

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-Guide-Puro

    Plasmid

    #86708

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Christoph Bock

    Article

    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcw107

    Plasmid

    #62511

    Purpose

    Gateway Expression Vector

    Depositor

    John Doench, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS Omomyc

    Plasmid

    #113164

    Purpose

    Omomyc expression in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

    Insert

    Omomyc (MYC Human)
    

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-TurboID-3MYC-natMX6 (pFB1434)

    Plasmid

    #126050

    Purpose

    For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LAMP1-APEX2

    Plasmid

    #215555

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMP1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid

    #129415

    Purpose

    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    RAB18 HDR template (RAB18 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0043-PspCas13b-gRNA non target

    Plasmid

    #223698

    Purpose

    gRNA control for dPspCas13b-FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for luciferase control
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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