167,564 results

1. AdEasier-1 cells (strain)

   Bacterial Strain

   #16399

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Bacterial Resistance

   Ampicillin and streptomycin

   Species

   Escherichia coli

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

2. 3xnls-mNeonGreen

   Plasmid

   #98875

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   3xNLS
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE (AAV5)

   Viral Prep

   #125713-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE (#125713). In addition to the viral particles, you will also receive purified pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Promoter

   Syn

   Tags

   mScarlet (Cre-dependent)

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212829

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Promoter

   CAG, TetOn

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-NOD2-EGFP

   Plasmid

   #131207

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

   Insert

   NOD2 (NOD2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCGN-HCF-1 fl

   Plasmid

   #53309

   Purpose

   plasmid contains human HCFC1 full length gene with HA and c-myc tags

   Depositor

   Winship Herr

   Article

   Wilson et al Cell. 1993 Jul 16;74(1):115-25.

   Insert

   HCFC1 (HCFC1 Human)
   

   Tags

   HA and c-myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV-EcoEnv

   Plasmid

   #15802

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   MLV env (ecotropic)
   

   Expression

   Mammalian

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. PPRE X3-TK-luc

   Plasmid

   #1015

   Purpose

   PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

   Depositor

   Bruce Spiegelman

   Article

   Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

   Insert

   PPRE
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Nov. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PhoDer

    Plasmid

    #188473

    Purpose

    Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

    Depositor

    Gary Mo

    Article

    Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

    Insert

    PhoDer (GSDMD Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-10His-mStayGold (E138D)

    Plasmid

    #211363

    Purpose

    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    E138D

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)

    Viral Prep

    #51085-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Ting

    Promoter

    Syn

    Tags

    nuclear dTomato (physically separate, not a fusion protein)

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pLenti-CMV-Blast-PIP-FUCCI

    Plasmid

    #138715

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CAG-jGCaMP8m-WPRE (AAV9)

    Viral Prep

    #179255-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CAG

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

16. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. v3em-Cterm-PE2max-∆RNaseH-dualU6

    Plasmid

    #198735

    Purpose

    AAV genome encoding C-terminal PE2max and U6 expression cassettes

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    NpuC-CtermPE2max∆RNaseH
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN(CMV-copGFP-Luc2-Puro)

    Plasmid

    #80389

    Purpose

    To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Luc2-F2A-Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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