Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133) Citations (6)

Originally described in: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Walton RT, Christie KA, Whittaker MN, Kleinstiver BP Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
PubMed Journal

Articles Citing pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

Articles
Small-molecule compounds boost genome-editing efficiency of cytosine base editor. Zhao T, Li Q, Zhou C, Lv X, Liu H, Tu T, Tang N, Cheng Y, Liu X, Liu C, Zhao J, Song Z, Wang H, Li J, Gu F. Nucleic Acids Res. 2021 Sep 7;49(15):8974-8986. doi: 10.1093/nar/gkab645. PubMed
Multiplex base- and prime-editing with drive-and-process CRISPR arrays. Yuan Q, Gao X. Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1. PubMed

Associated Plasmids

Disease modeling by efficient genome editing using a near PAM-less base editor in vivo. Rosello M, Serafini M, Mignani L, Finazzi D, Giovannangeli C, Mione MC, Concordet JP, Del Bene F. Nat Commun. 2022 Jun 15;13(1):3435. doi: 10.1038/s41467-022-31172-z. PubMed
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
LMNA Co-Regulated Gene Expression as a Suitable Readout after Precise Gene Correction. Wang H, Krause A, Escobar H, Muthel S, Metzler E, Spuler S. Int J Mol Sci. 2022 Dec 8;23(24):15525. doi: 10.3390/ijms232415525. PubMed
PAM-flexible genome editing with an engineered chimeric Cas9. Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodriguez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P. Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y. PubMed

Associated Plasmids

If you have published an article using this material, please email us at [email protected] to have your article added to this page.