pMA122 - peel-1 negative selection
PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34873||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeWorm targeting
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMA122 - peel-1 negative selection was a gift from Erik Jorgensen (Addgene plasmid # 34873 ; http://n2t.net/addgene:34873 ; RRID:Addgene_34873)
For your References section:Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM. Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. 10.1038/nmeth.1865 PubMed 22290181