231,592 results

1. pMSP2N2

   Plasmid

   #29520

   Depositor

   Stephen Sligar

   Article

   Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

   Insert

   MSP2N2
   

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   tandem MSP; His-tag on N-terminus followed by spa…

   Available Since

   May 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

   Viral Prep

   #73178-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV1)

   Viral Prep

   #162378-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+) eGFP

   Plasmid

   #129020

   Purpose

   Expresses eGFP

   Depositor

   Jeremy Wilusz

   Article

   Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

   Plasmid

   #233217

   Purpose

   Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv 2023.10.24.563674

   Insert

   Anti-amyloid-β 40/42 nanobody (R3VQ)
   

   Tags

   Sortase tag, His tag

   Expression

   Mammalian

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-mt-mKeima

   Plasmid

   #131626

   Purpose

   lentiviral expression of mitochondrial mKeima for generation of stable cell lines

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. eeBxb1

   Plasmid

   #222339

   Purpose

   Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEVS104

   Plasmid

   #207393

   Purpose

   Conjugal helper plasmid

   Depositor

   Eric Stabb, Jason Peters, Edward Ruby

   Article

   Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTNS2

    Plasmid

    #64968

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTNS2

    Plasmid

    #64968

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124428

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 PRDM16

    Plasmid

    #15503

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 PRDM16

    Plasmid

    #15503

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPLICS Mt-ER Short P2A

    Plasmid

    #164108

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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