167,570 results

1. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ132B2.0

   Plasmid

   #99888

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS1089: mini-AAV.sgRNA.Nme2Cas9

   Plasmid

   #159536

   Purpose

   Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 with single guide RNA cassette
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IP GFP-NBR1

   Plasmid

   #38283

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Next to BRCA1 gene 1 protein (NBR1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-nbOcIgG-Tn5

   Plasmid

   #184285

   Purpose

   Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   New York Genome Center, Ivan Raimondi

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbOcIgG-Tn5
   

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4TO-H2B-SunTag24x_v1

   Plasmid

   #60911

   Purpose

   Enocdes a histone H2B fused to the SunTag for single molecule imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   H2B
   

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB533_15F11-sfGFP

   Plasmid

   #167526

   Purpose

   H4K20me1-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

   Insert

   15F11-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLViP-LifeAct-iRFP670

   Plasmid

   #229699

   Purpose

   Lentiviral expression of LifeAct-iRFP670 in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   Synthetic construct LifeAct-mTurquoise gene
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX307-VHL-3F-BirA

   Plasmid

   #220147

   Purpose

   Expresses VHL-BirA for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ142

    Plasmid

    #69294

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. σ2-mCherry

    Plasmid

    #186579

    Purpose

    Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP2S1 (AP2S1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEmax_AAVS1_knockin_HDR_donor

    Plasmid

    #223000

    Purpose

    HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PEmax
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SNAP-23

    Plasmid

    #186686

    Purpose

    Expresses SNAP-23 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    SNAP23
    

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

    Plasmid

    #209283

    Purpose

    Expresses Voltron1 and jGCaMP7f in the zebrafish heart

    Depositor

    Adam Cohen

    Article

    Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

    Insert

    Voltron1_P2A_jGCaMP7f
    

    Use

    Zebrafish expression

    Promoter

    zACNE20_myl7

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXB1-nbMmIgG1-Tn5

    Plasmid

    #184287

    Purpose

    Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    New York Genome Center, Ivan Raimondi

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbMmIgG1-Tn5
    

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-MEG3

    Plasmid

    #44727

    Depositor

    Anne Klibanski

    Article

    Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

    Insert

    Maternally Expressed Gene 3 (MEG3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Flag FKHR

    Plasmid

    #13507

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR (FOXO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_TRAP1_WT_V5

    Plasmid

    #82994

    Purpose

    Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    TRAP1 (TRAP1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

    Plasmid

    #197044

    Purpose

    In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

    Depositor

    Liqun Luo

    Article

    Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

    Inserts

    pCAG-FSF-LSL
    SP-HA-HRPtm
    WPRE-bGHpolyA
    

    Tags

    HA, myc

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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