Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pUC57-sgRNA expression vector Citations (100)

Originally described in: Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
PubMed Journal

Articles Citing pUC57-sgRNA expression vector

An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Zhu X, Xu Y, Yu S, Lu L, Ding M, Cheng J, Song G, Gao X, Yao L, Fan D, Meng S, Zhang X, Hu S, Tian Y. Sci Rep. 2014 Sep 19;4:6420. doi: 10.1038/srep06420. PubMed
Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. PubMed

Associated Plasmids

Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system. Quadros RM, Harms DW, Ohtsuka M, Gurumurthy CB. FEBS Open Bio. 2015 Mar 10;5:191-7. doi: 10.1016/j.fob.2015.03.003. eCollection 2015. PubMed
GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice. Takahashi G, Gurumurthy CB, Wada K, Miura H, Sato M, Ohtsuka M. Sci Rep. 2015 Jun 22;5:11406. doi: 10.1038/srep11406. PubMed
CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA. Miura H, Gurumurthy CB, Sato T, Sato M, Ohtsuka M. Sci Rep. 2015 Aug 5;5:12799. doi: 10.1038/srep12799. PubMed
Preliminary Characterization of a Leptin Receptor Knockout Rat Created by CRISPR/Cas9 System. Bao D, Ma Y, Zhang X, Guan F, Chen W, Gao K, Qin C, Zhang L. Sci Rep. 2015 Nov 5;5:15942. doi: 10.1038/srep15942. PubMed
Regulator of G-Protein Signaling 10 Negatively Regulates Cardiac Remodeling by Blocking Mitogen-Activated Protein Kinase-Extracellular Signal-Regulated Protein Kinase 1/2 Signaling. Miao R, Lu Y, Xing X, Li Y, Huang Z, Zhong H, Huang Y, Chen AF, Tang X, Li H, Cai J, Yuan H. Hypertension. 2016 Jan;67(1):86-98. doi: 10.1161/HYPERTENSIONAHA.115.05957. Epub 2015 Nov 16. PubMed
Ubiquitin-Specific Protease 4 Is an Endogenous Negative Regulator of Pathological Cardiac Hypertrophy. He B, Zhao YC, Gao LC, Ying XY, Xu LW, Su YY, Ji QQ, Lin N, Pu J. Hypertension. 2016 Jun;67(6):1237-48. doi: 10.1161/HYPERTENSIONAHA.116.07392. Epub 2016 Apr 4. PubMed
Pivotal Role of Regulator of G-protein Signaling 12 in Pathological Cardiac Hypertrophy. Huang J, Chen L, Yao Y, Tang C, Ding J, Fu C, Li H, Ma G. Hypertension. 2016 Jun;67(6):1228-36. doi: 10.1161/HYPERTENSIONAHA.115.06877. Epub 2016 Apr 18. PubMed
Increasing the efficiency of CRISPR/Cas9-mediated precise genome editing in rats by inhibiting NHEJ and using Cas9 protein. Ma Y, Chen W, Zhang X, Yu L, Dong W, Pan S, Gao S, Huang X, Zhang L. RNA Biol. 2016 Jul 2;13(7):605-612. Epub 2016 May 10. PubMed
Regulator of G protein signalling 14 attenuates cardiac remodelling through the MEK-ERK1/2 signalling pathway. Li Y, Tang XH, Li XH, Dai HJ, Miao RJ, Cai JJ, Huang ZJ, Chen AF, Xing XW, Lu Y, Yuan H. Basic Res Cardiol. 2016 Jul;111(4):47. doi: 10.1007/s00395-016-0566-1. Epub 2016 Jun 13. PubMed
Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system. Bevacqua RJ, Fernandez-Martin R, Savy V, Canel NG, Gismondi MI, Kues WA, Carlson DF, Fahrenkrug SC, Niemann H, Taboga OA, Ferraris S, Salamone DF. Theriogenology. 2016 Nov;86(8):1886-1896.e1. doi: 10.1016/j.theriogenology.2016.06.010. Epub 2016 Jun 15. PubMed
Novel Protective Role for Ubiquitin-Specific Protease 18 in Pathological Cardiac Remodeling. Ying X, Zhao Y, Yao T, Yuan A, Xu L, Gao L, Ding S, Ding H, Pu J, He B. Hypertension. 2016 Nov;68(5):1160-1170. doi: 10.1161/HYPERTENSIONAHA.116.07562. Epub 2016 Aug 29. PubMed
Dual specific phosphatase 12 ameliorates cardiac hypertrophy in response to pressure overload. Li WM, Zhao YF, Zhu GF, Peng WH, Zhu MY, Yu XJ, Chen W, Xu DC, Xu YW. Clin Sci (Lond). 2017 Jan 1;131(2):141-154. doi: 10.1042/CS20160664. Epub 2016 Oct 4. PubMed
Smad Nuclear Interacting Protein 1 Acts as a Protective Regulator of Pressure Overload-Induced Pathological Cardiac Hypertrophy. Lu YY, Xu DC, Zhao YF, Zhu GF, Zhu MY, Liu WJ, Yu XJ, Chen W, Liu Z, Xu YW. J Am Heart Assoc. 2016 Oct 26;5(11). pii: e003943. PubMed
Variants of genes encoding collagens and matrix metalloproteinase system increased the risk of aortic dissection. Li Z, Zhou C, Tan L, Chen P, Cao Y, Li C, Li X, Yan J, Zeng H, Wang DW, Wang DW. Sci China Life Sci. 2017 Jan;60(1):57-65. doi: 10.1007/s11427-016-0333-3. Epub 2016 Dec 13. PubMed
ZNF307 (Zinc Finger Protein 307) Acts as a Negative Regulator of Pressure Overload-Induced Cardiac Hypertrophy. Yu CJ, Liang C, Li YX, Hu QQ, Zheng WW, Niu N, Yang X, Wang ZR, Yu XD, Zhang BL, Song BL, Zhang ZR. Hypertension. 2017 Apr;69(4):615-624. doi: 10.1161/HYPERTENSIONAHA.116.08500. Epub 2017 Feb 21. PubMed
Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy. Tang K, Zhao Y, Li H, Zhu M, Li W, Liu W, Zhu G, Xu D, Peng W, Xu YW. J Am Heart Assoc. 2017 Apr 21;6(4). pii: e004346. doi: 10.1161/JAHA.116.004346. PubMed
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y, Fan S, Jiang X, Khan T, Hao Q, Xu P, Nadano D, Huleihel M, Lunenfeld E, Wang PJ, Zhang Y, Shi Q. Curr Biol. 2017 May 22;27(10):1498-1505.e6. doi: 10.1016/j.cub.2017.04.017. Epub 2017 May 11. PubMed
Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Xu J, Xu X, Wang B, Ma Y, Zhang L, Xu H, Hu Y, Wu J, Cao X. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8620-8625. doi: 10.1073/pnas.1700917114. Epub 2017 Jul 24. PubMed
CRISPR/Cas9-mediated targeting of the Rosa26 locus produces Cre reporter rat strains for monitoring Cre-loxP-mediated lineage tracing. Ma Y, Yu L, Pan S, Gao S, Chen W, Zhang X, Dong W, Li J, Zhou R, Huang L, Han Y, Bai L, Zhang L, Zhang L. FEBS J. 2017 Oct;284(19):3262-3277. doi: 10.1111/febs.14188. Epub 2017 Sep 19. PubMed
Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis. Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, Qi M, Lu Z, Shi H, Wang J, Cheng Y, Luo G, Dai Q, Liu M, Guo X, Sha J, Shen B, He C. Cell Res. 2017 Sep;27(9):1115-1127. doi: 10.1038/cr.2017.99. Epub 2017 Aug 15. PubMed
Building Cre Knockin Rat Lines Using CRISPR/Cas9. Ma Y, Zhang L, Huang X. Methods Mol Biol. 2017;1642:37-52. doi: 10.1007/978-1-4939-7169-5_3. PubMed
MicroRNA-302a suppresses influenza A virus-stimulated interferon regulatory factor-5 expression and cytokine storm induction. Chen X, Zhou L, Peng N, Yu H, Li M, Cao Z, Lin Y, Wang X, Li Q, Wang J, She Y, Zhu C, Lu M, Zhu Y, Liu S. J Biol Chem. 2017 Dec 29;292(52):21291-21303. doi: 10.1074/jbc.M117.805937. Epub 2017 Oct 18. PubMed
Zinc-finger protein 418 overexpression protects against cardiac hypertrophy and fibrosis. Pan L, Sheng M, Huang Z, Zhu Z, Xu C, Teng L, He L, Gu C, Yi C, Li J. PLoS One. 2017 Oct 24;12(10):e0186635. doi: 10.1371/journal.pone.0186635. eCollection 2017. PubMed
i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases. Ohtsuka M, Sato M, Miura H, Takabayashi S, Matsuyama M, Koyano T, Arifin N, Nakamura S, Wada K, Gurumurthy CB. Genome Biol. 2018 Feb 26;19(1):25. doi: 10.1186/s13059-018-1400-x. PubMed
Abnormalities of hair structure and skin histology derived from CRISPR/Cas9-based knockout of phospholipase C-delta 1 in mice. Liu YM, Liu W, Jia JS, Chen BZ, Chen HW, Liu Y, Bie YN, Gu P, Sun Y, Xiao D, Gu WW. J Transl Med. 2018 May 25;16(1):141. doi: 10.1186/s12967-018-1512-9. PubMed
The transcription factor SOX30 is a key regulator of mouse spermiogenesis. Zhang D, Xie D, Lin X, Ma L, Chen J, Zhang D, Wang Y, Duo S, Feng Y, Zheng C, Jiang B, Ning Y, Han C. Development. 2018 May 30;145(11). pii: 145/11/dev164723. doi: 10.1242/dev.164723. PubMed
Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia. Kim MY, Yu KR, Kenderian SS, Ruella M, Chen S, Shin TH, Aljanahi AA, Schreeder D, Klichinsky M, Shestova O, Kozlowski MS, Cummins KD, Shan X, Shestov M, Bagg A, Morrissette JJD, Sekhri P, Lazzarotto CR, Calvo KR, Kuhns DB, Donahue RE, Behbehani GK, Tsai SQ, Dunbar CE, Gill S. Cell. 2018 May 31;173(6):1439-1453.e19. doi: 10.1016/j.cell.2018.05.013. Epub 2018 May 31. PubMed
Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes. Bai S, Fu K, Yin H, Cui Y, Yue Q, Li W, Cheng L, Tan H, Liu X, Guo Y, Zhang Y, Xie J, He W, Wang Y, Feng H, Xin C, Zhang J, Lin M, Shen B, Sun Z, Guo X, Zheng K, Ye L. Development. 2018 Jul 4;145(13). pii: dev.164855. doi: 10.1242/dev.164855. PubMed
Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang Y, Chen J, Sun Q, Huang X. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. PubMed

Associated Plasmids

Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy. Tokuhiro K, Dean J. Dev Cell. 2018 Sep 10;46(5):627-640.e5. doi: 10.1016/j.devcel.2018.07.020. Epub 2018 Aug 16. PubMed
Correction of the Marfan Syndrome Pathogenic FBN1 Mutation by Base Editing in Human Cells and Heterozygous Embryos. Zeng Y, Li J, Li G, Huang S, Yu W, Zhang Y, Chen D, Chen J, Liu J, Huang X. Mol Ther. 2018 Nov 7;26(11):2631-2637. doi: 10.1016/j.ymthe.2018.08.007. Epub 2018 Aug 14. PubMed
CRISPR-delivery particles targeting nuclear receptor-interacting protein 1 (Nrip1) in adipose cells to enhance energy expenditure. Shen Y, Cohen JL, Nicoloro SM, Kelly M, Yenilmez B, Henriques F, Tsagkaraki E, Edwards YJK, Hu X, Friedline RH, Kim JK, Czech MP. J Biol Chem. 2018 Nov 2;293(44):17291-17305. doi: 10.1074/jbc.RA118.004554. Epub 2018 Sep 6. PubMed
Paraoxonase 1 knockout rats have impaired T cell development at the CD4/CD8 double-negative to double-positive transition stage. Bai L, Shi G, Ma Y, Zhang L, Guan F, Zhang X, Xu Y, Chen H, Zhang L. Sci Rep. 2018 Sep 27;8(1):14457. doi: 10.1038/s41598-018-32780-w. PubMed
Targeted deletion of Insm2 in mice result in reduced insulin secretion and glucose intolerance. Wang L, Sun ZS, Xiang B, Wei CJ, Wang Y, Sun K, Chen G, Lan MS, Carmona GN, Notkins AL, Cai T. J Transl Med. 2018 Oct 25;16(1):297. doi: 10.1186/s12967-018-1665-6. PubMed
m(6)A facilitates hippocampus-dependent learning and memory through YTHDF1. Shi H, Zhang X, Weng YL, Lu Z, Liu Y, Lu Z, Li J, Hao P, Zhang Y, Zhang F, Wu Y, Delgado JY, Su Y, Patel MJ, Cao X, Shen B, Huang X, Ming GL, Zhuang X, Song H, He C, Zhou T. Nature. 2018 Nov;563(7730):249-253. doi: 10.1038/s41586-018-0666-1. Epub 2018 Oct 31. PubMed
Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells. Zhang T, Chen P, Li W, Sha S, Wang Y, Yuan Z, Shen B, Chen L. Glia. 2019 Apr;67(4):688-702. doi: 10.1002/glia.23565. Epub 2018 Nov 28. PubMed
Microbiota-Derived Lactate Accelerates Intestinal Stem-Cell-Mediated Epithelial Development. Lee YS, Kim TY, Kim Y, Lee SH, Kim S, Kang SW, Yang JY, Baek IJ, Sung YH, Park YY, Hwang SW, O E, Kim KS, Liu S, Kamada N, Gao N, Kweon MN. Cell Host Microbe. 2018 Dec 12;24(6):833-846.e6. doi: 10.1016/j.chom.2018.11.002. PubMed
PKG1-modified TSC2 regulates mTORC1 activity to counter adverse cardiac stress. Ranek MJ, Kokkonen-Simon KM, Chen A, Dunkerly-Eyring BL, Vera MP, Oeing CU, Patel CH, Nakamura T, Zhu G, Bedja D, Sasaki M, Holewinski RJ, Van Eyk JE, Powell JD, Lee DI, Kass DA. Nature. 2019 Feb;566(7743):264-269. doi: 10.1038/s41586-019-0895-y. Epub 2019 Jan 30. PubMed
Novel role of mitochondrial GTPases 1 in pathological cardiac hypertrophy. Xu D, Zhao Y, Weng X, Lu Y, Li W, Tang K, Chen W, Liu Z, Qi X, Zheng J, Fassett J, Zhang Y, Xu Y. J Mol Cell Cardiol. 2019 Mar;128:105-116. doi: 10.1016/j.yjmcc.2019.01.025. Epub 2019 Jan 29. PubMed
Label-Free LC-MS/MS Proteomics Analyses Reveal Proteomic Changes Accompanying MSTN KO in C2C12 Cells. Wang L, Huang Y, Wang X, Chen Y. Biomed Res Int. 2019 Apr 3;2019:7052456. doi: 10.1155/2019/7052456. eCollection 2019. PubMed
Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase. Yuan Z, Chen P, Zhang T, Shen B, Chen L. Cells. 2019 Jun 6;8(6). pii: cells8060552. doi: 10.3390/cells8060552. PubMed
Base pair editing in goat: nonsense codon introgression into FGF5 results in longer hair. Li G, Zhou S, Li C, Cai B, Yu H, Ma B, Huang Y, Ding Y, Liu Y, Ding Q, He C, Zhou J, Wang Y, Zhou G, Li Y, Yan Y, Hua J, Petersen B, Jiang Y, Sonstegard T, Huang X, Chen Y, Wang X. FEBS J. 2019 Jul 5. doi: 10.1111/febs.14983. PubMed
Efficient Generation of Pathogenic A-to-G Mutations in Human Tripronuclear Embryos via ABE-Mediated Base Editing. Li G, Liu X, Huang S, Zeng Y, Yang G, Lu Z, Zhang Y, Ma X, Wang L, Huang X, Liu J. Mol Ther Nucleic Acids. 2019 Jun 5;17:289-296. doi: 10.1016/j.omtn.2019.05.021. PubMed
P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice. Liu G, Wang Y, Zheng W, Cheng H, Zhou R. Int J Biol Sci. 2019 May 20;15(7):1383-1395. doi: 10.7150/ijbs.33773. eCollection 2019. PubMed
Hepatectomy promotes recurrence of liver cancer by enhancing IL-11-STAT3 signaling. Wang D, Zheng X, Fu B, Nian Z, Qian Y, Sun R, Tian Z, Wei H. EBioMedicine. 2019 Aug;46:119-132. doi: 10.1016/j.ebiom.2019.07.058. Epub 2019 Jul 30. PubMed
Improving Editing Efficiency for the Sequences with NGH PAM Using xCas9-Derived Base Editors. Liu X, Li G, Zhou X, Qiao Y, Wang R, Tang S, Liu J, Wang L, Huang X. Mol Ther Nucleic Acids. 2019 Sep 6;17:626-635. doi: 10.1016/j.omtn.2019.06.024. Epub 2019 Jul 12. PubMed
Roles of the hypoximir microRNA-424/322 in acute hypoxia and hypoxia-induced pulmonary vascular leakage. Tsai SH, Huang PH, Tsai HY, Hsu YJ, Chen YW, Wang JC, Chen YH, Lin SJ. FASEB J. 2019 Nov;33(11):12565-12575. doi: 10.1096/fj.201900564RR. Epub 2019 Aug 28. PubMed
Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex. Chen P, Zhang T, Yuan Z, Shen B, Chen L. Mol Brain. 2019 Aug 28;12(1):74. doi: 10.1186/s13041-019-0496-6. PubMed
Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Roesler MK, Lombino FL, Freitag S, Schweizer M, Hermans-Borgmeyer I, Schwarz JR, Kneussel M, Wagner W. Front Cell Neurosci. 2019 Aug 13;13:330. doi: 10.3389/fncel.2019.00330. eCollection 2019. PubMed
A DNAH17 missense variant causes flagella destabilization and asthenozoospermia. Zhang B, Ma H, Khan T, Ma A, Li T, Zhang H, Gao J, Zhou J, Li Y, Yu C, Bao J, Ali A, Murtaza G, Yin H, Gao Q, Jiang X, Zhang F, Liu C, Khan I, Zubair M, Hussain HMJ, Khan R, Yousaf A, Yuan L, Lu Y, Xu X, Wang Y, Tao Q, Hao Q, Fang H, Cheng H, Zhang Y, Shi Q. J Exp Med. 2020 Feb 3;217(2). pii: jem.20182365. doi: 10.1084/jem.20182365. PubMed
Homeostatic Function of Dermokine in the Skin Barrier and Inflammation. Utsunomiya A, Chino T, Utsunomiya N, Luong VH, Tokuriki A, Naganuma T, Arita M, Higashi K, Saito K, Suzuki N, Ohara A, Sugai M, Sugawara K, Tsuruta D, Oyama N, Hasegawa M. J Invest Dermatol. 2019 Oct 25. pii: S0022-202X(19)33303-2. doi: 10.1016/j.jid.2019.09.011. PubMed
Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis. Xiong Z, Xia P, Zhu X, Geng J, Wang S, Ye B, Qin X, Qu Y, He L, Fan D, Du Y, Tian Y, Fan Z. J Exp Med. 2020 Feb 3;217(2). pii: jem.20190974. doi: 10.1084/jem.20190974. PubMed
Diurnal oscillations of endogenous H2O2 sustained by p66(Shc) regulate circadian clocks. Pei JF, Li XK, Li WQ, Gao Q, Zhang Y, Wang XM, Fu JQ, Cui SS, Qu JH, Zhao X, Hao DL, Ju D, Liu N, Carroll KS, Yang J, Zhang EE, Cao JM, Chen HZ, Liu DP. Nat Cell Biol. 2019 Dec;21(12):1553-1564. doi: 10.1038/s41556-019-0420-4. Epub 2019 Nov 25. PubMed
Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys. Luo X, He Y, Zhang C, He X, Yan L, Li M, Hu T, Hu Y, Jiang J, Meng X, Ji W, Zhao X, Zheng P, Xu S, Su B. Nat Commun. 2019 Dec 4;10(1):5525. doi: 10.1038/s41467-019-13481-y. PubMed
CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice. Zuo Z, Ji MY, Zhao K, Su ZP, Li P, Hou DR, Li Y. Oxid Med Cell Longev. 2019 Nov 19;2019:7121763. doi: 10.1155/2019/7121763. eCollection 2019. PubMed
CTCF modulates allele-specific sub-TAD organization and imprinted gene activity at the mouse Dlk1-Dio3 and Igf2-H19 domains. Lleres D, Moindrot B, Pathak R, Piras V, Matelot M, Pignard B, Marchand A, Poncelet M, Perrin A, Tellier V, Feil R, Noordermeer D. Genome Biol. 2019 Dec 12;20(1):272. doi: 10.1186/s13059-019-1896-8. PubMed
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation. Wang X, Liu Z, Li G, Dang L, Huang S, He L, Ma Y, Li C, Liu M, Yang G, Huang X, Zhou F, Ma X. Mol Ther. 2020 Feb 5;28(2):431-440. doi: 10.1016/j.ymthe.2019.11.022. Epub 2019 Nov 29. PubMed
Skeletal deterioration in COL2A1-related spondyloepiphyseal dysplasia occurs prior to osteoarthritis. Rolvien T, Yorgan TA, Kornak U, Hermans-Borgmeyer I, Mundlos S, Schmidt T, Niemeier A, Schinke T, Amling M, Oheim R. Osteoarthritis Cartilage. 2020 Mar;28(3):334-343. doi: 10.1016/j.joca.2019.12.011. Epub 2020 Jan 17. PubMed
CRLS1 ameliorates nonalcoholic steatohepatitis through ATF3 transcriptional inactivation. Tu C, Xiong H, Hu Y, Wang W, Mei G, Wang H, Li Y, Zhou Z, Meng F, Zhang P, Mei Z. Hepatology. 2020 Feb 25. doi: 10.1002/hep.31202. PubMed
Quantitative live imaging of Venus::BMAL1 in a mouse model reveals complex dynamics of the master circadian clock regulator. Yang N, Smyllie NJ, Morris H, Goncalves CF, Dudek M, Pathiranage DRJ, Chesham JE, Adamson A, Spiller DG, Zindy E, Bagnall J, Humphreys N, Hoyland J, Loudon ASI, Hastings MH, Meng QJ. PLoS Genet. 2020 Apr 30;16(4):e1008729. doi: 10.1371/journal.pgen.1008729. eCollection 2020 Apr. PubMed
Alopecia areata susceptibility variant in MHC region impacts expressions of genes contributing to hair keratinization and is involved in hair loss. Oka A, Takagi A, Komiyama E, Yoshihara N, Mano S, Hosomichi K, Suzuki S, Haida Y, Motosugi N, Hatanaka T, Kimura M, Ueda MT, Nakagawa S, Miura H, Ohtsuka M, Tanaka M, Komiyama T, Otomo A, Hadano S, Mabuchi T, Beck S, Inoko H, Ikeda S. EBioMedicine. 2020 Jul;57:102810. doi: 10.1016/j.ebiom.2020.102810. Epub 2020 Jun 21. PubMed
Zebrafish IL-4-like Cytokines and IL-10 Suppress Inflammation but Only IL-10 Is Essential for Gill Homeostasis. Bottiglione F, Dee CT, Lea R, Zeef LAH, Badrock AP, Wane M, Bugeon L, Dallman MJ, Allen JE, Hurlstone AFL. J Immunol. 2020 Aug 15;205(4):994-1008. doi: 10.4049/jimmunol.2000372. Epub 2020 Jul 8. PubMed
NULP1 Alleviates Cardiac Hypertrophy by Suppressing NFAT3 Transcriptional Activity. Zhang X, Lei F, Wang XM, Deng KQ, Ji YX, Zhang Y, Li H, Zhang XD, Lu Z, Zhang P. J Am Heart Assoc. 2020 Aug 18;9(16):e016419. doi: 10.1161/JAHA.120.016419. Epub 2020 Aug 4. PubMed
A single copy of large tumor suppressor 1 or large tumor suppressor 2 is sufficient for normal hematopoiesis. Li ZG, Fu XM, Chai CY, Sun FF, Xiao FF, Huang YX, Yao K, Chen JP, Hou Y. Chin Med J (Engl). 2020 Aug 20;133(16):1943-1951. doi: 10.1097/CM9.0000000000000934. PubMed
Congestive heart failure in COX2 deficient rats. Wan Q, Kong D, Liu Q, Guo S, Wang C, Zhao Y, Ke ZJ, Yu Y. Sci China Life Sci. 2020 Sep 14. pii: 10.1007/s11427-020-1792-5. doi: 10.1007/s11427-020-1792-5. PubMed
FBXW5 acts as a negative regulator of pathological cardiac hypertrophy by decreasing the TAK1 signaling to pro-hypertrophic members of the MAPK signaling pathway. Hui X, Hu F, Liu J, Li C, Yang Y, Shu S, Liu P, Wang F, Li S. J Mol Cell Cardiol. 2021 Feb;151:31-43. doi: 10.1016/j.yjmcc.2020.09.008. Epub 2020 Sep 22. PubMed
MicroRNA-210 regulates placental adaptation to maternal hypoxic stress during pregnancydagger. Bian X, Liu J, Yang Q, Liu Y, Jia W, Zhang X, Li YX, Shao X, Wang YL. Biol Reprod. 2021 Feb 11;104(2):418-429. doi: 10.1093/biolre/ioaa187. PubMed
A Cas-embedding strategy for minimizing off-target effects of DNA base editors. Liu Y, Zhou C, Huang S, Dang L, Wei Y, He J, Zhou Y, Mao S, Tao W, Zhang Y, Yang H, Huang X, Chi T. Nat Commun. 2020 Nov 27;11(1):6073. doi: 10.1038/s41467-020-19690-0. PubMed
Multiplex gene targeting in the mouse embryo using a Cas9-Cpf1 hybrid guide RNA. Oh SH, Lee HJ, Ahn MK, Jeon MY, Yoon JS, Jung YJ, Kim GN, Baek IJ, Kim I, Kim KM, Sung YH. Biochem Biophys Res Commun. 2021 Feb 5;539:48-55. doi: 10.1016/j.bbrc.2020.12.072. Epub 2021 Jan 6. PubMed
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe. Liu C, Gao J, Cui X, Li Z, Chen L, Yuan Y, Zhang Y, Mei L, Zhao L, Cai D, Hu M, Zhou B, Li Z, Qin T, Si H, Li G, Lin Z, Xu Y, Zhu C, Yin Y, Zhang C, Xu W, Li Q, Wang K, Gilbert MTP, Heller R, Wang W, Huang J, Qiu Q. Sci Adv. 2021 Mar 17;7(12). pii: 7/12/eabe9459. doi: 10.1126/sciadv.abe9459. Print 2021 Mar. PubMed
LITAF acts as a novel regulator for pathological cardiac hypertrophy. Xiang M, Yang F, Zhou Y, Li W, Zou Y, Ye P, Zhu L, Wang PX, Chen M. J Mol Cell Cardiol. 2021 Jul;156:82-94. doi: 10.1016/j.yjmcc.2021.03.012. Epub 2021 Apr 3. PubMed
Mutations in RNA Methyltransferase Gene NSUN5 Confer High Risk of Outflow Tract Malformation. Wang Y, Jiang T, Xu J, Gu Y, Zhou Y, Lin Y, Wu Y, Li W, Wang C, Shen B, Mo X, Wang X, Zhou B, Ding C, Hu Z. Front Cell Dev Biol. 2021 Apr 21;9:623394. doi: 10.3389/fcell.2021.623394. eCollection 2021. PubMed
Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes. Liu X, Zhou X, Li G, Huang S, Sun W, Sun Q, Li L, Huang X, Liu J, Wang L. CRISPR J. 2021 Oct;4(5):710-727. doi: 10.1089/crispr.2021.0036. PubMed
Role of GALNT4 in protecting against cardiac hypertrophy through ASK1 signaling pathway. Zhang BB, Gao L, Yang Q, Liu Y, Yu XY, Shen JH, Zhang WC, Han ZY, Chen SZ, Guo S. Cell Death Dis. 2021 Oct 22;12(11):980. doi: 10.1038/s41419-021-04222-5. PubMed
Lysosomal-Associated Protein Transmembrane 5 Functions as a Novel Negative Regulator of Pathological Cardiac Hypertrophy. Gao L, Guo S, Long R, Xiao L, Yao R, Zheng X, Zhang Y, Wang X. Front Cardiovasc Med. 2021 Oct 6;8:740526. doi: 10.3389/fcvm.2021.740526. eCollection 2021. PubMed
Uncoupling protein 1 knockout aggravates isoproterenol-induced acute myocardial ischemia via AMPK/mTOR/PPARalpha pathways in rats. Hou D, Fu H, Zheng Y, Lu D, Ma Y, Yin Y, Zhang L, Bao D. Transgenic Res. 2022 Feb;31(1):107-118. doi: 10.1007/s11248-021-00289-0. Epub 2021 Oct 28. PubMed
SMPX Deficiency Causes Stereocilia Degeneration and Progressive Hearing Loss in CBA/CaJ Mice. Tu H, Zhang A, Fu X, Xu S, Bai X, Wang H, Gao J. Front Cell Dev Biol. 2021 Oct 14;9:750023. doi: 10.3389/fcell.2021.750023. eCollection 2021. PubMed
TRIM34 modulates influenza virus-activated programmed cell death by targeting Z-DNA-binding protein 1 for K63-linked polyubiquitination. Wang X, Xiong J, Zhou D, Zhang S, Wang L, Tian Q, Li C, Liu J, Wu Y, Li J, Wang J. J Biol Chem. 2022 Mar;298(3):101611. doi: 10.1016/j.jbc.2022.101611. Epub 2022 Jan 20. PubMed
Knockout of Stearoyl-CoA Desaturase 1 Decreased Milk Fat and Unsaturated Fatty Acid Contents of the Goat Model Generated by CRISPR/Cas9. Tian H, Niu H, Luo J, Yao W, Chen X, Wu J, Geng Y, Gao W, Lei A, Gao Z, Tian X, Zhao X, Shi H, Li C, Hua J. J Agric Food Chem. 2022 Apr 6;70(13):4030-4043. doi: 10.1021/acs.jafc.2c00642. Epub 2022 Mar 28. PubMed
TRDMT1 exhibited protective effects against LPS-induced inflammation in rats through TLR4-NF-kappaB/MAPK-TNF-alpha pathway. Li Z, Qi X, Zhang X, Yu L, Gao L, Kong W, Chen W, Dong W, Luo L, Lu D, Zhang L, Ma Y. Animal Model Exp Med. 2022 Apr;5(2):172-182. doi: 10.1002/ame2.12221. PubMed
DEC1 represses cardiomyocyte hypertrophy by recruiting PRP19 as an E3 ligase to promote ubiquitination-proteasome-mediated degradation of GATA4. Cheng L, Xu C, Chen Y, Li J, Wang J, Xiao J, Ma M, Feng X, Wei X, Fang J. J Mol Cell Cardiol. 2022 Aug;169:96-110. doi: 10.1016/j.yjmcc.2022.05.005. Epub 2022 May 31. PubMed
CCL17 acts as a novel therapeutic target in pathological cardiac hypertrophy and heart failure. Zhang Y, Ye Y, Tang X, Wang H, Tanaka T, Tian R, Yang X, Wang L, Xiao Y, Hu X, Jin Y, Pang H, Du T, Liu H, Sun L, Xiao S, Dong R, Ferrucci L, Tian Z, Zhang S. J Exp Med. 2022 Aug 1;219(8). pii: 213274. doi: 10.1084/jem.20200418. Epub 2022 Jun 10. PubMed
MicroRNA-202 safeguards meiotic progression by preventing premature SEPARASE-mediated REC8 cleavage. Chen J, Gao C, Luo M, Zheng C, Lin X, Ning Y, Ma L, He W, Xie D, Liu K, Hong K, Han C. EMBO Rep. 2022 Aug 3;23(8):e54298. doi: 10.15252/embr.202154298. Epub 2022 Jun 17. PubMed
Lysosomal-associated transmembrane protein 5 deficiency exacerbates cerebral ischemia/reperfusion injury. Zhang Z, Wang L, Wang Z, Zhang T, Shi M, Xin C, Zou Y, Wei W, Li X, Chen J, Zhao W. Front Mol Neurosci. 2022 Aug 15;15:971361. doi: 10.3389/fnmol.2022.971361. eCollection 2022. PubMed
Generation of genetically engineered mice for lung cancer with mutant EGFR. Kim DS, Ji W, Kim DH, Choi YJ, Im K, Lee CW, Cho J, Min J, Woo DC, Choi CM, Lee JC, Sung YH, Rho JK. Biochem Biophys Res Commun. 2022 Dec 3;632:85-91. doi: 10.1016/j.bbrc.2022.09.104. Epub 2022 Sep 30. PubMed
E3 ubiquitin ligase RNF5 attenuates pathological cardiac hypertrophy through STING. Yang LL, Xiao WC, Li H, Hao ZY, Liu GZ, Zhang DH, Wu LM, Wang Z, Zhang YQ, Huang Z, Zhang YZ. Cell Death Dis. 2022 Oct 21;13(10):889. doi: 10.1038/s41419-022-05231-8. PubMed
RNA-methyltransferase Nsun5 controls the maternal-to-zygotic transition by regulating maternal mRNA stability. Ding C, Lu J, Li J, Hu X, Liu Z, Su H, Li H, Huang B. Clin Transl Med. 2022 Dec;12(12):e1137. doi: 10.1002/ctm2.1137. PubMed
Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress. Ghanam AR, Ke S, Wang S, Elgendy R, Xie C, Wang S, Zhang R, Wei M, Liu W, Cao J, Zhang Y, Zhang Z, Xue T, Zheng Y, Song X. iScience. 2022 Dec 5;26(1):105740. doi: 10.1016/j.isci.2022.105740. eCollection 2023 Jan 20. PubMed
Scd1 Deficiency in Early Embryos Affects Blastocyst ICM Formation through RPs-Mdm2-p53 Pathway. Niu H, Lei A, Tian H, Yao W, Liu Y, Li C, An X, Chen X, Zhang Z, Wu J, Yang M, Huang J, Cheng F, Zhao J, Hua J, Liu S, Luo J. Int J Mol Sci. 2023 Jan 16;24(2):1750. doi: 10.3390/ijms24021750. PubMed
YTHDF1 Attenuates TBI-Induced Brain-Gut Axis Dysfunction in Mice. Huang P, Liu M, Zhang J, Zhong X, Zhong C. Int J Mol Sci. 2023 Feb 20;24(4):4240. doi: 10.3390/ijms24044240. PubMed
Impaired bone strength and bone microstructure in a novel early-onset osteoporotic rat model with a clinically relevant PLS3 mutation. Hu J, Zhou B, Lin X, Zhang Q, Guan F, Sun L, Liu J, Wang O, Jiang Y, Xia WB, Xing X, Li M. Elife. 2023 Apr 21;12:e80365. doi: 10.7554/eLife.80365. PubMed
Single-cell RNA-seq uncovers dynamic processes orchestrated by RNA-binding protein DDX43 in chromatin remodeling during spermiogenesis. Tan H, Wang W, Zhou C, Wang Y, Zhang S, Yang P, Guo R, Chen W, Zhang J, Ye L, Cui Y, Ni T, Zheng K. Nat Commun. 2023 Apr 29;14(1):2499. doi: 10.1038/s41467-023-38199-w. PubMed
Lysosomal-associated protein transmembrane 5 ameliorates non-alcoholic steatohepatitis by promoting the degradation of CDC42 in mice. Jiang L, Zhao J, Yang Q, Li M, Liu H, Xiao X, Tian S, Hu S, Liu Z, Yang P, Chen M, Ye P, Xia J. Nat Commun. 2023 May 8;14(1):2654. doi: 10.1038/s41467-023-37908-9. PubMed
High-Altitude Andean H194R HIF2A Allele Is a Hypomorphic Allele. Jorgensen K, Song D, Weinstein J, Garcia OA, Pearson LN, Inclan M, Rivera-Chira M, Leon-Velarde F, Kiyamu M, Brutsaert TD, Bigham AW, Lee FS. Mol Biol Evol. 2023 Jul 5;40(7):msad162. doi: 10.1093/molbev/msad162. PubMed
TRAF Family Member 4 Promotes Cardiac Hypertrophy Through the Activation of the AKT Pathway. Li J, Wang CQ, Xiao WC, Chen Y, Tu J, Wan F, Deng KQ, Li HP. J Am Heart Assoc. 2023 Sep 5;12(17):e028185. doi: 10.1161/JAHA.122.028185. Epub 2023 Aug 29. PubMed
GC-biased gene conversion drives accelerated evolution of ultraconserved elements in mammalian and avian genomes. Liu A, Wang N, Xie G, Li Y, Yan X, Li X, Zhu Z, Li Z, Yang J, Meng F, Dou M, Chen W, Ma N, Jiang Y, Gao Y, Wang Y. Genome Res. 2023 Oct;33(10):1673-1689. doi: 10.1101/gr.277784.123. Epub 2023 Oct 26. PubMed
Phase-separated CCER1 coordinates the histone-to-protamine transition and male fertility. Qin D, Gu Y, Zhang Y, Wang S, Jiang T, Wang Y, Wang C, Chen C, Zhang T, Xu W, Wang H, Zhang K, Hu L, Li L, Xie W, Wu X, Hu Z. Nat Commun. 2023 Dec 11;14(1):8209. doi: 10.1038/s41467-023-43480-z. PubMed
Prime editing in mice with an engineered pegRNA. Salem AR, Bryant WB, Doja J, Griffin SH, Shi X, Han W, Su Y, Verin AD, Miano JM. Vascul Pharmacol. 2024 Mar;154:107269. doi: 10.1016/j.vph.2023.107269. Epub 2023 Dec 27. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.