Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGL3-U6-sgRNA-BFP
(Plasmid #107722)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107722 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

The depositing laboratory states that the sequences of BFP and GFP are very similar, and the difference is the 66th amino acid. GFP contains Tyr (Y), while BFP contains His (H). This plasmid contains the H at position 66

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-U6-sgRNA-BFP was a gift from Xingxu Huang (Addgene plasmid # 107722 ; http://n2t.net/addgene:107722 ; RRID:Addgene_107722)
  • For your References section:

    Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang Y, Chen J, Sun Q, Huang X. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. 10.1038/s41467-018-04768-7 PubMed 29904106