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Dane Wittrup Lab Plasmids


The Dane Wittrup Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Isolating and engineering human antibodies using yeast surface display. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD Nat Protoc. 2006;1(2):755-68.
Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup KD J Mol Biol. 2004 Oct 22;343(3):685-701.
Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. Colby DW, Chu Y, Cassady JP, Duennwald M, Zazulak H, Webster JM, Messer A, Lindquist S, Ingram VM, Wittrup KD Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17616-21. Epub 2004 Dec 14.
Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Lipovsek D, Antipov E, Armstrong KA, Olsen MJ, Klibanov AM, Tidor B, Wittrup KD Chem Biol. 2007 Oct;14(10):1176-85.
Yeast surface display for screening combinatorial polypeptide libraries. Boder ET, Wittrup KD Nat Biotechnol. 1997 Jun;15(6):553-7.
Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. Hackel BJ, Kapila A, Wittrup KD J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24.
Yeast Secretion Vectors (unpublished)
Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast. Mata-Fink J, Kriegsman B, Yu HX, Zhu H, Hanson MC, Irvine DJ, Wittrup KD J Mol Biol. 2013 Jan 23;425(2):444-56. doi: 10.1016/j.jmb.2012.11.010. Epub 2012 Nov 15.
Targeted cytolysins synergistically potentiate cytoplasmic delivery of gelonin immunotoxin. Pirie CM, Liu DV, Wittrup KD Mol Cancer Ther. 2013 Sep;12(9):1774-82. doi: 10.1158/1535-7163.MCT-12-1023. Epub 2013 Jul 5.
Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. Pirie CM, Hackel BJ, Rosenblum MG, Wittrup KD J Biol Chem. 2011 Feb 11;286(6):4165-72. doi: 10.1074/jbc.M110.186973. Epub 2010 Dec 7.
Synergistic Innate and Adaptive Immune Response to Combination Immunotherapy with Anti-Tumor Antigen Antibodies and Extended Serum Half-Life IL-2. Zhu EF, Gai SA, Opel CF, Kwan BH, Surana R, Mihm MC, Kauke MJ, Moynihan KD, Angelini A, Williams RT, Stephan MT, Kim JS, Yaffe MB, Irvine DJ, Weiner LM, Dranoff G, Wittrup KD Cancer Cell. 2015 Apr 13;27(4):489-501. doi: 10.1016/j.ccell.2015.03.004.
Isolation of anti-T cell receptor scFv mutants by yeast surface display. Kieke MC, Cho BK, Boder ET, Kranz DM, Wittrup KD Protein Eng. 1997 Nov;10(11):1303-10.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
41843pCTcon2CD20Yeast ExpressionIsolating and engineering human antibodies using yeast surface display. Nat Protoc. 2006;1(2):755-68. pCT Add to Cart
41844pCT-4M5.34M5.3 (Synthetic)Yeast ExpressionSubstantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. J Mol Biol. 2004 Oct 22;343(3):685-701. pCT
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on backbone)
  • Add to Cart
    41845pCT3024-4-20 (Synthetic)Yeast ExpressionSubstantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. J Mol Biol. 2004 Oct 22;343(3):685-701. pCT
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on backbone)
  • Add to Cart
    42900pCT-L7.5.1L7.5.1Yeast ExpressionPicomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24. pCT Add to Cart
    43862pNES-VL12.3VL12.3 (Synthetic)Mammalian ExpressionPotent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17616-21. Epub 2004 Dec 14. pcDNA3.1(-)Zeo Add to Cart
    44021pCT2-HRPHRP (Synthetic)Yeast ExpressionSelection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Chem Biol. 2007 Oct;14(10):1176-85. pCTcon2 Add to Cart
    44036pIU211AGA1Yeast ExpressionYeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol. 1997 Jun;15(6):553-7. YIplac211 Add to Cart
    44874pCHA-VRC01-scFvVRC01 scFvYeast ExpressionRapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast. J Mol Biol. 2013 Jan 23;425(2):444-56. doi: 10.1016/j.jmb.2012.11.010. Epub 2012 Nov 15. pCHA Add to Cart
    45830pRS314-4M5.34M5.3Yeast Expression Yeast Secretion Vectors (unpublished) pRS314 Add to Cart
    45831pRS316-4M5.34M5.3Yeast Expression Yeast Secretion Vectors (unpublished) pRS316 Add to Cart
    51280pMal-E4rGelE4rGelBacterial ExpressionConvergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. J Biol Chem. 2011 Feb 11;286(6):4165-72. doi: 10.1074/jbc.M110.186973. Epub 2010 Dec 7. Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria pMal Add to Cart
    51281pMal-E6LLOE6LLOBacterial ExpressionTargeted cytolysins synergistically potentiate cytoplasmic delivery of gelonin immunotoxin. Mol Cancer Ther. 2013 Sep;12(9):1774-82. doi: 10.1158/1535-7163.MCT-12-1023. Epub 2013 Jul 5. Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria pMal Add to Cart
    65419FcD265A-IL2FcD265A-IL2 (Mus musculus)Mammalian ExpressionSynergistic Innate and Adaptive Immune Response to Combination Immunotherapy with Anti-Tumor Antigen Antibodies and Extended Serum Half-Life IL-2. Cancer Cell. 2015 Apr 13;27(4):489-501. doi: 10.1016/j.ccell.2015.03.004. Mammalian expression plasmid for murine serum-persistent IL-2 gWIZ Il2 Il-2 Add to Cart
    65420FcD265A-FLAGFcD265A-FLAG (Mus musculus)Mammalian ExpressionSynergistic Innate and Adaptive Immune Response to Combination Immunotherapy with Anti-Tumor Antigen Antibodies and Extended Serum Half-Life IL-2. Cancer Cell. 2015 Apr 13;27(4):489-501. doi: 10.1016/j.ccell.2015.03.004. Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production gWIZ Add to Cart
    73447pYD1Yeast ExpressionIsolation of anti-T cell receptor scFv mutants by yeast surface display. Protein Eng. 1997 Nov;10(11):1303-10. expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells pYD1
  • Tags / Fusion Proteins
    • AGA2 (N terminal on backbone)
    • T7 peptide (N terminal on backbone)
    • Xpress tag (N terminal on backbone)
    • V5 tag (C terminal on backbone)
    • 6xHis (C terminal on backbone)
  • Add to Cart