Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

Dane Wittrup Lab Plasmids


The Dane Wittrup Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

Learn more about research in the Dane Wittrup Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

Title Authors Publication
Targeted cytolysins synergistically potentiate cytoplasmic delivery of gelonin immunotoxin. Pirie CM, Liu DV, Wittrup KD Mol Cancer Ther. 2013 Sep;12(9):1774-82. doi: 10.1158/1535-7163.MCT-12-1023. Epub 2013 Jul 5.
Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast. Mata-Fink J, Kriegsman B, Yu HX, Zhu H, Hanson MC, Irvine DJ, Wittrup KD J Mol Biol. 2013 Jan 23;425(2):444-56. doi: 10.1016/j.jmb.2012.11.010. Epub 2012 Nov 15.
Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. Pirie CM, Hackel BJ, Rosenblum MG, Wittrup KD J Biol Chem. 2011 Feb 11;286(6):4165-72. doi: 10.1074/jbc.M110.186973. Epub 2010 Dec 7.
Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. Hackel BJ, Kapila A, Wittrup KD J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24.
Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Lipovsek D, Antipov E, Armstrong KA, Olsen MJ, Klibanov AM, Tidor B, Wittrup KD Chem Biol. 2007 Oct;14(10):1176-85.
Isolating and engineering human antibodies using yeast surface display. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD Nat Protoc. 2006;1(2):755-68.
Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. Colby DW, Chu Y, Cassady JP, Duennwald M, Zazulak H, Webster JM, Messer A, Lindquist S, Ingram VM, Wittrup KD Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17616-21. Epub 2004 Dec 14.
Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup KD J Mol Biol. 2004 Oct 22;343(3):685-701.
Yeast surface display for screening combinatorial polypeptide libraries. Boder ET, Wittrup KD Nat Biotechnol. 1997 Jun;15(6):553-7.
Yeast Secretion Vectors (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
41843 pCTcon2 CD20 Yeast Expression Isolating and engineering human antibodies using yeast surface display. Nat Protoc. 2006;1(2):755-68. pCT Add to Cart
41844 pCT-4M5.3 4M5.3 (Synthetic) Yeast Expression Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. J Mol Biol. 2004 Oct 22;343(3):685-701. pCT
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on backbone)
  • Add to Cart
    41845 pCT302 4-4-20 (Synthetic) Yeast Expression Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. J Mol Biol. 2004 Oct 22;343(3):685-701. pCT
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on backbone)
  • Add to Cart
    42900 pCT-L7.5.1 L7.5.1 Yeast Expression Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24. pCT Add to Cart
    43862 pNES-VL12.3 VL12.3 (Synthetic) Mammalian Expression Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17616-21. Epub 2004 Dec 14. pcDNA3.1(-)Zeo Add to Cart
    44021 pCT2-HRP HRP (Synthetic) Yeast Expression Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Chem Biol. 2007 Oct;14(10):1176-85. pCTcon2 Add to Cart
    44036 pIU211 AGA1 Yeast Expression Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol. 1997 Jun;15(6):553-7. YIplac211 Add to Cart
    44874 pCHA-VRC01-scFv VRC01 scFv Yeast Expression Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast. J Mol Biol. 2013 Jan 23;425(2):444-56. doi: 10.1016/j.jmb.2012.11.010. Epub 2012 Nov 15. pCHA Add to Cart
    45830 pRS314-4M5.3 4M5.3 Yeast Expression Yeast Secretion Vectors (unpublished) pRS314 Add to Cart
    45831 pRS316-4M5.3 4M5.3 Yeast Expression Yeast Secretion Vectors (unpublished) pRS316 Add to Cart
    51280 pMal-E4rGel E4rGel Bacterial Expression Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. J Biol Chem. 2011 Feb 11;286(6):4165-72. doi: 10.1074/jbc.M110.186973. Epub 2010 Dec 7. Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria pMal Add to Cart
    51281 pMal-E6LLO E6LLO Bacterial Expression Targeted cytolysins synergistically potentiate cytoplasmic delivery of gelonin immunotoxin. Mol Cancer Ther. 2013 Sep;12(9):1774-82. doi: 10.1158/1535-7163.MCT-12-1023. Epub 2013 Jul 5. Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria pMal Add to Cart