Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases.
Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
Nat Biotechnol. 2009 Aug 13. ():. PubMed Article
Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
Nat Biotechnol. 2009 Aug 13. ():. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 22072 | AAVS1 hPGK-PuroR-pA donor | |
| 22073 | TV-hPITX3-forward | |
| 22074 | pTRE-TIGHT-EGFP-donor fw copy | |
| 22075 | AAVS1 SA-2A-puro-pA donor | AAV zinc finger donor vector for genomic targeting |
| 22077 | TRE-TIGHT-EGFP-backward donor | |
| 22208 | TV-hPITX3-Reverse | |
| 22209 | OCT4 GFP puro Donor 1 | |
| 22210 | OCT4 GFP puro Donor 2 | |
| 22211 | OCT4 GFP puro Donor 3 | |
| 22212 | AAV-CAGGS-EGFP | AAV zinc finger donor vector for genomic targeting |