pcDNA3 Flag FKHR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13507||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3 Flag
Backbone manufacturermade in Guan Lab
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneFOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
See author's map for more information on cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 Flag FKHR was a gift from Kunliang Guan (Addgene plasmid # 13507 ; http://n2t.net/addgene:13507 ; RRID:Addgene_13507)
For your References section:Negative regulation of the forkhead transcription factor FKHR by Akt. Tang ED, Nunez G, Barr FG, Guan KL. J Biol Chem. 1999 Jun 11. 274(24):16741-6. 10.1074/jbc.274.24.16741 PubMed 10358014