165,637 results

1. pHCMV_DENV1_PrME

   Plasmid

   #207263

   Purpose

   Mammalian expression of Dengue virus 1 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV1_PrME (POLY Dengue virus 1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-Ii_neomycin

   Plasmid

   #65312

   Purpose

   Cytoplasmic ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Invariant chain (p33)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Streptavidin in its cytoplasmid part

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL1425 (pSPIN, pCDF backbone)

   Plasmid

   #160735

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdestMB14_sur5p-dCas9-VP64

   Plasmid

   #177788

   Purpose

   Overexpression of dCas9-VP64 in C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   dCas9-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEvol-pAzFRS.2.t1

   Plasmid

   #73546

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.2.t1
   pAzFRS.2.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-puro largeTcDNA

   Plasmid

   #14088

   Depositor

   William Hahn

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   Large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. BL21ΔABCF

   Bacterial Strain

   #102270

   Purpose

   ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   Tetracycline

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

8. bactin2:loxP-STOP-loxP-DsRed-express

   Plasmid

   #68797

   Purpose

   For making transgenic fish

   Depositor

   David Traver

   Article

   Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

   Inserts

   beta-actin promoter
   loxp-stop-codon-loxp-dsred
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine (AAV8)

   Viral Prep

   #50455-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine (#50455). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, inhibitory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCitrine (Cre-dependent)

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6M-Pak1

    Plasmid

    #12209

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-HA-deLACCO1-NGR

    Plasmid

    #208024

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBlueSKMimRNA

    Plasmid

    #102535

    Purpose

    Template for synthesis of Minos transposase mRNA

    Depositor

    Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transposase mRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLZts

    Plasmid

    #128799

    Purpose

    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

    Depositor

    Timothy Barnett

    Article

    Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

    Type

    Empty backbone

    Use

    Bacterial mutagenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

    Viral Prep

    #198516-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    EF-1a

    Tags

    mScarlet

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES2-EGFP Mer

    Plasmid

    #14998

    Depositor

    Raymond Birge

    Article

    Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

    Insert

    Mer (Mertk Mouse)
    

    Use

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-TFRC

    Plasmid

    #172420

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDestTol2pACryGFP

    Plasmid

    #64022

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    GFP
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    alpha-Acrystallin promoter

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge