163,421 results

1. CTCF-mAC donor (Hygro)

   Plasmid

   #140646

   Purpose

   CTCF tagging with mAID-clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti Lifeact-mRuby2 PuroR

   Plasmid

   #84386

   Purpose

   Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

   Plasmid

   #203840

   Purpose

   Kir2.1 expression for silencing neurons and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   2A-mScarlet-KASH

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mAID-BRD4 donor

   Plasmid

   #140650

   Purpose

   BRD4 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIGneo

   Plasmid

   #52116

   Purpose

   a bicistronic IRES-GFP containing vector for mammalian expression (CMV promoter)

   Depositor

   Dario Vignali

   Type

   Empty backbone

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+-eGFP

   Plasmid

   #221710

   Purpose

   Expression of eGFP in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   eGFP
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-CMV-Galpha-13 ONE-GO

   Plasmid

   #189740

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-13 ONE-GO
   GNA13 (GNA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L342M (present in YFP but no functional consequen…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-hMC4R

    Plasmid

    #179315

    Purpose

    Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    melanocortin 4 receptor (MC4R Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-DN-hCUL4B-FLAG

    Plasmid

    #15822

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4B (CUL4B Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N594

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-DN-hCUL5-FLAG

    Plasmid

    #15823

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N441

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ223 (dAsCpf1-SRDX)

    Plasmid

    #86209

    Purpose

    Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    dAsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    NLS and SRDX repressor and NLS

    Expression

    Plant

    Mutation

    D908A

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin2 (CUL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Use

    Tags

    GCaMP3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NanoLuc-PM

    Plasmid

    #164784

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    

    Use

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV Retrograde)

    Viral Prep

    #104495-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    CAG

    Tags

    None

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA 3.1 SIRT1-FL

    Plasmid

    #105670

    Purpose

    Mammalian expression construct of mouse SIRT1 full length isoform

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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