163,765 results

1. T-2A-EGFP-PGK-Puro

   Plasmid

   #83344

   Purpose

   Donor vector to knocin EGFP into human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   Human Brachyury knockin homology arms (TBXT Human)
   

   Use

   Tags

   2A-EGFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

   Plasmid

   #113899

   Purpose

   Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   TetR-HA-NLS-P2A-BSD-Myc
   

   Use

   Lentiviral

   Tags

   HA-Myc

   Expression

   Mutation

   Promoter

   CMV-MIE

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-NLS-actin

   Plasmid

   #118380

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   NLS

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6 x DBE-luc

   Plasmid

   #153160

   Purpose

   Luciferase reporter plasmid for FOXTF

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   minP

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-tomato SOX2-sgRNA

   Plasmid

   #196190

   Purpose

   Editing human SOX2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. FoxO1-ADA-GFP

   Plasmid

   #35640

   Depositor

   Domenico Accili

   Article

   Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-4

   Insert

   FoxO1-ADA-GFP (Foxo1 Mouse)
   

   Use

   Tags

   Flag, GFP, and Myc

   Expression

   Mammalian

   Mutation

   T24A, S253D and S316A mutations

   Promoter

   CMV

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBad-mKalama1

   Plasmid

   #14892

   Purpose

   Bacterial expression of mKalama1 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   mKalama1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pBI-p53 R175H/EGFP

   Plasmid

   #16544

   Depositor

   Bert Vogelstein

   Article

   Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R175H

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-ALK

   Plasmid

   #116712

   Purpose

   Lentiviral expression of ALK

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ALK (ALK Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMYT017

    Plasmid

    #180670

    Purpose

    Encodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

    Depositor

    Tom Ellis

    Article

    Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

    Insert

    LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPROBE-gfp[LVA]

    Plasmid

    #40170

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotor probe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. c-myc promoter (TBE 1/2 - wt)

    Plasmid

    #16595

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (TBE 1/2 wt) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pHAGE-EGFR-G719S

    Plasmid

    #116256

    Purpose

    Lentiviral expression of EGFR G719S

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G719S

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HTR4-Tango

    Plasmid

    #66412

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR4 (HTR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217341

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-Bst HF

    Plasmid

    #165539

    Purpose

    High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Bst HF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCB59 pDEST crybb1:mKate2

    Plasmid

    #195986

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEQ176

    Plasmid

    #83943

    Purpose

    express beta-galactosidase in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Schleiss et al J Virol. 1991 Dec;65(12):6782-9.

    Insert

    CMV enhancer/promoter
    

    Use

    Tags

    beta-galactosidase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid

    #75140

    Purpose

    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 91-710

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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