167,924 results

1. pGEX-2TK 14-3-3 sigma GST

   Plasmid

   #11944

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 sigma (SFN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pAUX_OL

   Plasmid

   #166246

   Purpose

   use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
   

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and BBa_J23116

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOBI C-FLAG PHGDH

   Plasmid

   #212995

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2932

   Plasmid

   #144408

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAP2A (TFAP2A Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6x-His_A1aY1_GFP

   Plasmid

   #158753

   Purpose

   Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   6xHistidine_A1aY1_GFP
   

   Tags

   6x-Histidine tag, GFP, T7 leader sequence, and Th…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hs.NRAS Q61R

   Plasmid

   #83179

   Purpose

   Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

   Depositor

   Dominic Esposito

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61R

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426_Cas9_gRNA-ARS208a

   Plasmid

   #87383

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ITPR1_Halo_N_allele

   Plasmid

   #178161

   Purpose

   Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (ITPR1 Human)
   

   Use

   Donor vector

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-DIO-SPOTlight-U2GCR

    Plasmid

    #231889

    Purpose

    Cre-dependent reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Insert

    DIO-SPOTlight-U2GCR
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid

    #26429

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. E. coli K-12 MG1655_rLP5(nth-ydgR)

    Bacterial Strain

    #110245

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pJK511

    Plasmid

    #155384

    Purpose

    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. RP156 pUAST EGFP:Gr21a

    Plasmid

    #59524

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Gr21 (Gr21a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pPBT-peRNA_GG-Puro

    Plasmid

    #173220

    Purpose

    piggyBac transposon containing hU6 promotor and pegRNA cloning site

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    pegRNA cloning cassette
    PuroR
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1-a and hU6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_YWHAZ_14-3-3

    Plasmid

    #109808

    Purpose

    Protein expression and purification of YWHAZ_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAZ_14-3-3 (YWHAZ Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-L1-ESYN-L4

    Plasmid

    #32580

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    Enhanced Synapsin Promoter
    

    Use

    Gateway cloning vector

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSC5

    Plasmid

    #104779

    Purpose

    pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 5KKP (PUS7)

    Plasmid

    #101878

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PUS7 (PUS7 Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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