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pEGFP-hGal3
(Plasmid #73080)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73080 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5460
  • Modifications to backbone
    No major modification to backbone. Digested with Bgl1+Sal1 to add the insert cDNA.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Galectin-3
  • Alt name
    LGALS3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    754
  • Entrez Gene
    LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl2 (not destroyed)
  • 3′ cloning site Sal1 (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is used to detect ruptured lysosomal membrane by lysosomotropic agents or endosomal membrane accompanied with invading bacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hGal3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 73080 ; http://n2t.net/addgene:73080 ; RRID:Addgene_73080)
  • For your References section:

    Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury. Maejima I, Takahashi A, Omori H, Kimura T, Takabatake Y, Saitoh T, Yamamoto A, Hamasaki M, Noda T, Isaka Y, Yoshimori T. EMBO J. 2013 Aug 28;32(17):2336-47. doi: 10.1038/emboj.2013.171. Epub 2013 Aug 6. 10.1038/emboj.2013.171 PubMed 23921551