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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Publication

Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Publication

Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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lenti sgRNA(MS2)_puro backbone

Plasmid

#73795

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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lenti sgRNA(MS2)_puro backbone

Plasmid

#73795

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

134 Articles

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

134 Articles

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pET His6 MBP TEV LIC cloning vector (1M)

Plasmid

#29656

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pET His6 MBP TEV LIC cloning vector (1M)

Plasmid

#29656

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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AAVS1 SA-2A-puro-pA donor

Plasmid

#22075

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-Puro

Use

Zfn donor

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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AAVS1 SA-2A-puro-pA donor

Plasmid

#22075

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-Puro

Use

Zfn donor

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Publication

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Publication

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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EZ-Tet-pLKO-Blast

Plasmid

#85973

Purpose

Tet/Dox inducible shRNA lentivirus with blasticidin selection

Depositor

Cindy Miranti

Publication

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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EZ-Tet-pLKO-Blast

Plasmid

#85973

Purpose

Tet/Dox inducible shRNA lentivirus with blasticidin selection

Depositor

Cindy Miranti

Publication

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

IRES-EGFP

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

IRES-EGFP

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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