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EZ-Tet-pLKO-Blast
(Plasmid #85973)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85973 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 85973-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EZ-Tet-pLKO-Puro
  • Backbone size (bp) 8985
  • Modifications to backbone
    Cut out PuroR gene with BamHI and AatII and PCR subcloned in BlastR.
  • Vector type
    Lentiviral, RNAi
  • Promoter H1 / TetO
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

Resource Information

Information for Cloning Grade DNA (Catalog # 85973-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EZ-Tet-pLKO-Blast was a gift from Cindy Miranti (Addgene plasmid # 85973 ; http://n2t.net/addgene:85973 ; RRID:Addgene_85973)
  • For your References section:

    A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector. Frank SB, Schulz VV, Miranti CK. BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x. 10.1186/s12896-017-0341-x [pii] PubMed 28245848