Purpose(Empty Backbone) Tet/Dox inducible shRNA lentivirus with puromycin selection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85966||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||85966-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Backbone manufacturerDmitri Wiederschain
- Backbone size (bp) 10633
Modifications to backboneShrunk stuffer and changed 5' cloning site from AgeI to NheI.
Vector typeLentiviral, RNAi
- Promoter H1 / TetO
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberLow Copy
- 5′ sequencing primer ATTAGTGAACGGATCTCGACGG
- 3′ sequencing primer AACCCAGGGCTGCCTTGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Modified from Wiederschain et al., Addgene plasmid #21915
Information for Cloning Grade DNA (Catalog # 85966-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EZ-Tet-pLKO-Puro was a gift from Cindy Miranti (Addgene plasmid # 85966 ; http://n2t.net/addgene:85966 ; RRID:Addgene_85966)
For your References section:A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector. Frank SB, Schulz VV, Miranti CK. BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x. 10.1186/s12896-017-0341-x [pii] PubMed 28245848