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EZ-Tet-pLKO-Puro
(Plasmid #85966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85966 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Tet-pLKO-Puro
  • Backbone manufacturer
    Dmitri Wiederschain
  • Backbone size (bp) 10633
  • Modifications to backbone
    Shrunk stuffer and changed 5' cloning site from AgeI to NheI.
  • Vector type
    Lentiviral, RNAi
  • Promoter H1 / TetO
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modified from Wiederschain et al., Addgene plasmid #21915

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EZ-Tet-pLKO-Puro was a gift from Cindy Miranti (Addgene plasmid # 85966 ; http://n2t.net/addgene:85966 ; RRID:Addgene_85966)

  • For your References section:

    A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector. Frank SB, Schulz VV, Miranti CK. BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x. 10.1186/s12896-017-0341-x PubMed 28245848
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