Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pET His6 MBP TEV LIC cloning vector (1M) Citations (33)

Originally described in: LIC N-terminal fusion vectors for E. coli expression

Articles Citing pET His6 MBP TEV LIC cloning vector (1M)

Evolutionarily conserved roles of the dicer helicase domain in regulating RNA interference processing. Kidwell MA, Chan JM, Doudna JA. J Biol Chem. 2014 Oct 10;289(41):28352-62. doi: 10.1074/jbc.M114.589051. Epub 2014 Aug 18. PubMed
Cas9 specifies functional viral targets during CRISPR-Cas adaptation. Heler R, Samai P, Modell JW, Weiner C, Goldberg GW, Bikard D, Marraffini LA. Nature. 2015 Mar 12;519(7542):199-202. doi: 10.1038/nature14245. Epub 2015 Feb 18. PubMed
Histone deacetylase 6 structure and molecular basis of catalysis and inhibition. Hai Y, Christianson DW. Nat Chem Biol. 2016 Sep;12(9):741-7. doi: 10.1038/nchembio.2134. Epub 2016 Jul 25. PubMed

Associated Plasmids

Histone deacetylase 10 structure and molecular function as a polyamine deacetylase. Hai Y, Shinsky SA, Porter NJ, Christianson DW. Nat Commun. 2017 May 18;8:15368. doi: 10.1038/ncomms15368. PubMed
Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex. Clerici M, Faini M, Aebersold R, Jinek M. Elife. 2017 Dec 23;6. pii: 33111. doi: 10.7554/eLife.33111. PubMed
Hsp90 Mediates Membrane Deformation and Exosome Release. Lauwers E, Wang YC, Gallardo R, Van der Kant R, Michiels E, Swerts J, Baatsen P, Zaiter SS, McAlpine SR, Gounko NV, Rousseau F, Schymkowitz J, Verstreken P. Mol Cell. 2018 Sep 6;71(5):689-702.e9. doi: 10.1016/j.molcel.2018.07.016. PubMed
Molecular Basis for Autocatalytic Backbone N-Methylation in RiPP Natural Product Biosynthesis. Ongpipattanakul C, Nair SK. ACS Chem Biol. 2018 Oct 19;13(10):2989-2999. doi: 10.1021/acschembio.8b00668. Epub 2018 Sep 25. PubMed
Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules. Kuo YW, Trottier O, Mahamdeh M, Howard J. Proc Natl Acad Sci U S A. 2019 Mar 19;116(12):5533-5541. doi: 10.1073/pnas.1818824116. Epub 2019 Mar 5. PubMed
Molecular mechanism of the RNA helicase DHX37 and its activation by UTP14A in ribosome biogenesis. Boneberg FM, Brandmann T, Kobel L, van den Heuvel J, Bargsten K, Bammert L, Kutay U, Jinek M. RNA. 2019 Jun;25(6):685-701. doi: 10.1261/rna.069609.118. Epub 2019 Mar 25. PubMed
DNA-guided DNA cleavage at moderate temperatures by Clostridium butyricum Argonaute. Hegge JW, Swarts DC, Chandradoss SD, Cui TJ, Kneppers J, Jinek M, Joo C, van der Oost J. Nucleic Acids Res. 2019 May 9. pii: 5487266. doi: 10.1093/nar/gkz306. PubMed

Associated Plasmids

Argonaute bypasses cellular obstacles without hindrance during target search. Cui TJ, Klein M, Hegge JW, Chandradoss SD, van der Oost J, Depken M, Joo C. Nat Commun. 2019 Sep 26;10(1):4390. doi: 10.1038/s41467-019-12415-y. PubMed
Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes. Osko JD, Christianson DW. Methods Enzymol. 2019;626:447-474. doi: 10.1016/bs.mie.2019.06.028. Epub 2019 Jul 18. PubMed
Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes. Porter NJ, Christianson DW. Methods Enzymol. 2019;626:561-585. doi: 10.1016/bs.mie.2019.06.029. Epub 2019 Jul 18. PubMed
PD-L1 tumor-intrinsic signaling and its therapeutic implication in triple-negative breast cancer. Chen C, Li S, Xue J, Qi M, Liu X, Huang Y, Hu J, Dong H, Ling K. JCI Insight. 2021 Apr 22;6(8). pii: 131458. doi: 10.1172/jci.insight.131458. PubMed
Detecting m(6)A with In Vitro DART-Seq. Tegowski M, Zhu H, Meyer KD. Methods Mol Biol. 2022;2404:363-374. doi: 10.1007/978-1-0716-1851-6_20. PubMed
MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface. Houlard M, Cutts EE, Shamim MS, Godwin J, Weisz D, Presser Aiden A, Lieberman Aiden E, Schermelleh L, Vannini A, Nasmyth K. Elife. 2021 Dec 1;10. pii: 73348. doi: 10.7554/eLife.73348. PubMed
The YPhi motif defines the structure-activity relationships of human 20S proteasome activators. Opoku-Nsiah KA, de la Pena AH, Williams SK, Chopra N, Sali A, Lander GC, Gestwicki JE. Nat Commun. 2022 Mar 9;13(1):1226. doi: 10.1038/s41467-022-28864-x. PubMed
HSF2 cooperates with HSF1 to drive a transcriptional program critical for the malignant state. Smith RS, Takagishi SR, Amici DR, Metz K, Gayatri S, Alasady MJ, Wu Y, Brockway S, Taiberg SL, Khalatyan N, Taipale M, Santagata S, Whitesell L, Lindquist S, Savas JN, Mendillo ML. Sci Adv. 2022 Mar 18;8(11):eabj6526. doi: 10.1126/sciadv.abj6526. Epub 2022 Mar 16. PubMed
The Structure of the Arabidopsis PEX4-PEX22 Peroxin Complex-Insights Into Ubiquitination at the Peroxisomal Membrane. Traver MS, Bradford SE, Olmos JL Jr, Wright ZJ, Miller MD, Xu W, Phillips GN Jr, Bartel B. Front Cell Dev Biol. 2022 Feb 18;10:838923. doi: 10.3389/fcell.2022.838923. eCollection 2022. PubMed
Improved Methods for Deamination-Based m(6)A Detection. Zhu H, Yin X, Holley CL, Meyer KD. Front Cell Dev Biol. 2022 Apr 27;10:888279. doi: 10.3389/fcell.2022.888279. eCollection 2022. PubMed

Associated Plasmids

A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice. Malonis RJ, Georgiev GI, Haslwanter D, VanBlargan LA, Fallon G, Vergnolle O, Cahill SM, Harris R, Cowburn D, Chandran K, Diamond MS, Lai JR. PLoS Pathog. 2022 Jun 9;18(6):e1010573. doi: 10.1371/journal.ppat.1010573. eCollection 2022 Jun. PubMed
A glycine-rich PE_PGRS protein governs mycobacterial actin-based motility. Hill NS, Welch MD. Nat Commun. 2022 Jun 24;13(1):3608. doi: 10.1038/s41467-022-31333-0. PubMed
A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes. Borgo GM, Burke TP, Tran CJ, Lo NTN, Engstrom P, Welch MD. Nat Commun. 2022 Jun 27;13(1):3656. doi: 10.1038/s41467-022-31351-y. PubMed
HupZ, a Unique Heme-Binding Protein, Enhances Group A Streptococcus Fitness During Mucosal Colonization. Lyles KV, Thomas LS, Ouellette C, Cook LCC, Eichenbaum Z. Front Cell Infect Microbiol. 2022 Jun 14;12:867963. doi: 10.3389/fcimb.2022.867963. eCollection 2022. PubMed
Application of Aspergillus niger Fumonisin Amine Oxidase (AnFAO) to Detoxify Fumonisin-Contaminated Maize. Telmer PG, Kelman MJ, Renaud JB, Sumarah MW, Garnham CP. Toxins (Basel). 2022 Aug 9;14(8):544. doi: 10.3390/toxins14080544. PubMed
Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3' end biogenesis. Muckenfuss LM, Migenda Herranz AC, Boneberg FM, Clerici M, Jinek M. Elife. 2022 Sep 8;11:e80332. doi: 10.7554/eLife.80332. PubMed
Programming xenon diffusion in maltose-binding protein. Zhao Z, Rudman NA, He J, Dmochowski IJ. Biophys J. 2022 Dec 6;121(23):4635-4643. doi: 10.1016/j.bpj.2022.10.025. Epub 2022 Oct 20. PubMed
C-terminal determinants for RNA binding motif 7 protein stability and RNA recognition. Sobeh AM, Eichhorn CD. Biophys Chem. 2023 Jan;292:106928. doi: 10.1016/j.bpc.2022.106928. Epub 2022 Nov 8. PubMed
Programmable RNA detection with CRISPR-Cas12a. Jain P, Rananaware S, Vesco E, Shoemaker G, Anekar S, Sandoval LS, Meister K, Macaluso N, Nguyen L. Res Sq. 2023 Feb doi: 10.21203/ Preprint. PubMed
Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids. Nguyen LT, Rananaware SR, Yang LG, Macaluso NC, Ocana-Ortiz JE, Meister KS, Pizzano BLM, Sandoval LSW, Hautamaki RC, Fang ZR, Joseph SM, Shoemaker GM, Carman DR, Chang L, Rakestraw NR, Zachary JF, Guerra S, Perez A, Jain PK. Cell Rep Med. 2023 May 16;4(5):101037. doi: 10.1016/j.xcrm.2023.101037. Epub 2023 May 8. PubMed

Associated Plasmids

Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits. Grove DJ, Levine DJ, Kearse MG. Nucleic Acids Res. 2023 Oct 13;51(18):9983-10000. doi: 10.1093/nar/gkad683. PubMed
Structure and mechanism of the human CTDNEP1-NEP1R1 membrane protein phosphatase complex necessary to maintain ER membrane morphology. Gao S, Carrasquillo Rodriguez JW, Bahmanyar S, Airola MV. bioRxiv [Preprint]. 2023 Nov 20:2023.11.20.567952. doi: 10.1101/2023.11.20.567952. PubMed
Functional divergence of a bacterial enzyme promotes healthy or acneic skin. Hajam IA, Katiki M, McNally R, Lazaro-Diez M, Kolar S, Chatterjee A, Gonzalez C, Paulchakrabarti M, Choudhury B, Caldera JR, Desmond T, Tsai CM, Du X, Li H, Murali R, Liu GY. Nat Commun. 2023 Dec 5;14(1):8061. doi: 10.1038/s41467-023-43833-8. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.