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PurposeCRISPR-Cas9-assisted genome editing in Escherichia coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTargetF
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameccdB
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEcgRNA was a gift from Sheng Yang (Addgene plasmid # 166581 ; http://n2t.net/addgene:166581 ; RRID:Addgene_166581) -
For your References section:
A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S. Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627. doi: 10.1093/abbs/gmab036. 10.1093/abbs/gmab036 PubMed 33764372
