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(Plasmid #74448)


Item Catalog # Description Quantity Price (USD)
Plasmid 74448 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    H2O-forming NADH oxidase from Lactobacillus brevis
  • Species
    Lactobacillus brevis
  • Insert Size (bp)
  • Mutation
    Human codon optimized
  • Tags / Fusion Proteins
    • Flag tag with linker (C terminal on insert)
    • mitochondrial targeting sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Mlu1 (not destroyed)
  • 5′ sequencing primer GTAAAACGACGGCCAGTG
  • 3′ sequencing primer GGAAACAGCTATGACCATG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In our original publication we express mitoLbNOX in mammalian cells using a Tet3G dox-inducible system from Clontech. We cannot distribute those exact plasmids due to Clontech license restrictions. We recommend that investigators subclone this mitoLbNOX sequence into their preferred expression system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-mitoLbNOX was a gift from Vamsi Mootha (Addgene plasmid # 74448 ; ; RRID:Addgene_74448)
  • For your References section:

    Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK. Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. Epub 2016 Apr 7. 10.1126/science.aad4017 PubMed 27124460