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(Plasmid #75285)


Item Catalog # Description Quantity Price (USD)
Plasmid 75285 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    H2O-forming NADH oxidase from Lactobacillus brevis
  • Species
    Lactobacillus brevis
  • Insert Size (bp)
  • Mutation
    Human codon optimized
  • Tag / Fusion Protein
    • C-terminal FLAG tag with linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TGT AAA ACG ACG GCC AGT
  • 3′ sequencing primer CAG GAA ACA GCT ATG AC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In our original publication we express LbNOX in mammalian cells using a Tet3G dox-inducible system from Clontech. We cannot distribute those exact plasmids due to Clontech license restrictions. We recommend that investigators subclone this LbNOX sequence into their preferred expression system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-LbNOX was a gift from Vamsi Mootha (Addgene plasmid # 75285 ; ; RRID:Addgene_75285)
  • For your References section:

    Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK. Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. Epub 2016 Apr 7. 10.1126/science.aad4017 PubMed 27124460