PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||75285||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 4131
Growth in Bacteria
Copy numberHigh Copy
Alt nameH2O-forming NADH oxidase from Lactobacillus brevis
Insert Size (bp)1421
MutationHuman codon optimized
/ Fusion Protein
- C-terminal FLAG tag with linker (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer TGT AAA ACG ACG GCC AGT
- 3′ sequencing primer CAG GAA ACA GCT ATG AC (Common Sequencing Primers)
In our original publication we express LbNOX in mammalian cells using a Tet3G dox-inducible system from Clontech. We cannot distribute those exact plasmids due to Clontech license restrictions. We recommend that investigators subclone this LbNOX sequence into their preferred expression system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC57-LbNOX was a gift from Vamsi Mootha (Addgene plasmid # 75285 ; http://n2t.net/addgene:75285 ; RRID:Addgene_75285)
For your References section:Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK. Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. Epub 2016 Apr 7. 10.1126/science.aad4017 PubMed 27124460