167,601 results

1. pBabe-Puro-MEK-DD

   Plasmid

   #15268

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   Map2k1 (Mapk1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S218D S222D

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115268

   Purpose

   Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   EiCsm6
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Cyclin A2

   Plasmid

   #215048

   Purpose

   Expresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNA2 (CCNA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0756

   Plasmid

   #141685

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUND (JUND Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-UBF

   Plasmid

   #26672

   Depositor

   Sui Huang

   Article

   Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

   Insert

   UBF1 (UBTF Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

6. TFORF3232

   Plasmid

   #144708

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUNB (JUNB Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDrDERusk

   Plasmid

   #213703

   Purpose

   Vector for red-light-induced bacterial gene expression

   Depositor

   Andreas Moeglich

   Article

   Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mRhubarb720

   Plasmid

   #197212

   Purpose

   Expresses the protein of mRhubarb720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   mRhubarb720
   

   Use

   AAV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLexm

   Plasmid

   #99844

   Purpose

   mammalian expression

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-Flag-humanRBM47

    Plasmid

    #134607

    Purpose

    Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    Flag-humanRBM47 (RBM47 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMX/cyclinB1-GFP (27)

    Plasmid

    #26061

    Depositor

    Jonathon Pines

    Article

    Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-CMV-GFP-W

    Plasmid

    #196046

    Purpose

    lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Promoter

    CMV

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid

    #218514

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(1)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-AKAP-mtagBFP2-UBC6

    Plasmid

    #105007

    Purpose

    Mammalian Expression of mtagBFP2

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    mtagBFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAMA-YAP

    Plasmid

    #74942

    Purpose

    Lentiviral over-expression vector for YAP

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-mCherry

    Promoter

    EF1α

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTiGc

    Plasmid

    #78314

    Purpose

    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

    Depositor

    Samantha Sampson

    Article

    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

    Inserts

    TurboFP635
    GFP
    

    Use

    Synthetic Biology; Mycobacterial replicating vect…

    Expression

    Bacterial

    Promoter

    hsp60 and hsp60(ribo)

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pOpen-NotIR (GC)

    Plasmid

    #165507

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    NotI (GC-rich recognition site)
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRS314-T7-GFP

    Plasmid

    #33130

    Depositor

    Michael Rosbash

    Article

    Dower et al RNA. 2002 May;8(5):686-97.

    Insert

    GFP with TDH3 5' and 3' UTR (gfp )
    

    Expression

    Yeast

    Promoter

    T7

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. NES-Grx1-roGFP2

    Plasmid

    #236551

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    NES-Grx1-roGFP2 (NES Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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