169,099 results

1. pAG CMV Empty Puro

   Plasmid

   #237881

   Purpose

   Empty mammalian expression plasmid for cloning untagged proteins

   Depositor

   Meghan Rego, Addgene Research Program

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. eIF2a 2

   Plasmid

   #21808

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Expression

   Mammalian

   Mutation

   S52A

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-Streptavidin-Alive

   Plasmid

   #20860

   Purpose

   Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Alive
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Wt gene

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLO-Blue

   Plasmid

   #220782

   Purpose

   Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   blue chromoprotein (aeBlue)
   

   Tags

   8xHis

   Expression

   Bacterial

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP1122

   Plasmid

   #104159

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. eIF2a 3

   Plasmid

   #21809

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Expression

   Mammalian

   Mutation

   S52D

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

   Plasmid

   #127289

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

   Inserts

   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and Ascl1 linked via 2A

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSFFV_mNG3K(1-10)

   Plasmid

   #157993

   Purpose

   Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

   Depositor

   Bo Huang

   Article

   Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

   Insert

   pSFFV_mNG3K(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   G27D, D42E, M128I, K153M, T170I

   Promoter

   SFFV

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mCherry

   Plasmid

   #128744

   Purpose

   Expresses mCherry in mammalian cells

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-HA-OTUB1

    Plasmid

    #22551

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOG44_FLP_recombinase (pEHA1338)

    Plasmid

    #209087

    Purpose

    FLP recombinase for FRT-mediated integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Insert

    FLP recombinase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJC5-LAMP1-RFP-3xHA

    Plasmid

    #102932

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    RFP-HA

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFPGUSPlus

    Plasmid

    #64401

    Purpose

    binary plant vector expressing GFP and GUS, both driven by a 35S promoter

    Depositor

    Claudia Vickers

    Article

    Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

    Inserts

    GFP
    GUSPlus
    

    Use

    Binary vector for plant transformation

    Tags

    His

    Expression

    Plant

    Mutation

    S65T

    Promoter

    35S

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP_mNG2(11)_Clathrin light chain

    Plasmid

    #82608

    Purpose

    Expresses mNG2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNG2(11)_Clathrin light chain
    

    Expression

    Mammalian

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Su9-EGFP

    Plasmid

    #23214

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Subunit 9 of the F0-ATPase
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains AA 1-69 of subunit 9

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV2/9 REP-AAP-ΔCAP

    Plasmid

    #205991

    Purpose

    Rep and AAP protein expression for AAV CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    AAV9 Rep and AAP proteins for CREATE libraries
    

    Use

    AAV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. plentiCRISPRv2-sgEGFP

    Plasmid

    #86153

    Purpose

    Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBbi 41BBL-CD86 mIL-15

    Plasmid

    #183245

    Purpose

    Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Mutation

    D10A and H840A

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.3/TO/V5-DEST-EGFP

    Plasmid

    #215538

    Purpose

    Lentiviral transfer vector to express the EGFP fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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