|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50005||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||50005-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector backbonepUC6, Addgene plasmid #49793
Backbone manufacturerMessing Lab
- Backbone size (bp) 2686
Vector typecloning vector
- Promoter lac
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Identical to pUC18 (Addgene plasmid #50004) except that the multiple cloning sites (MCS) are arranged in opposite orientation.
Information for Cloning Grade DNA (Catalog # 50005-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC19 was a gift from Joachim Messing (Addgene plasmid # 50005 ; http://n2t.net/addgene:50005 ; RRID:Addgene_50005)
For your References section:Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Norrander J, Kempe T, Messing J. Gene. 1983 Dec;26(1):101-6. 10.1016/0378-1119(83)90040-9 PubMed 6323249