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pUC18
(Plasmid #50004)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50004 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC6, Addgene plasmid #49793
  • Backbone manufacturer
    Messing Lab
  • Backbone size (bp) 2680
  • Modifications to backbone
    polylinker from M13mp18 (Addgene plasmid #49986) added to polylinker.
  • Vector type
    Bacterial Expression
  • Promoter lac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Identical to pUC19 (Addgene plasmid #50005) except that the multiple cloning sites (MCS) are arranged in opposite orientation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18 was a gift from Joachim Messing (Addgene plasmid # 50004 ; http://n2t.net/addgene:50004 ; RRID:Addgene_50004)
  • For your References section:

    Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Norrander J, Kempe T, Messing J. Gene. 1983 Dec;26(1):101-6. 10.1016/0378-1119(83)90040-9 PubMed 6323249