|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50004||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepUC6, Addgene plasmid #49793
Backbone manufacturerMessing Lab
- Backbone size (bp) 2680
Modifications to backbonepolylinker from M13mp18 (Addgene plasmid #49986) added to polylinker.
Vector typeBacterial Expression
- Promoter lac
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
Identical to pUC19 (Addgene plasmid #50005) except that the multiple cloning sites (MCS) are arranged in opposite orientation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC18 was a gift from Joachim Messing (Addgene plasmid # 50004 ; http://n2t.net/addgene:50004 ; RRID:Addgene_50004)
For your References section:Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Norrander J, Kempe T, Messing J. Gene. 1983 Dec;26(1):101-6. 10.1016/0378-1119(83)90040-9 PubMed 6323249