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pcDNA3-EGFP-Cdc42(wt)
(Plasmid #12599)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12599 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-Cdc42 (WT)
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-EGFP-Cdc42(wt) was a gift from Klaus Hahn (Addgene plasmid # 12599 ; http://n2t.net/addgene:12599 ; RRID:Addgene_12599)
  • For your References section:

    Activation of endogenous Cdc42 visualized in living cells. Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM. Science. 2004 Sep 10. 305(5690):1615-9. 10.1126/science.1100367 PubMed 15361624