|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12599||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameEGFP-Cdc42 (WT)
SpeciesH. sapiens (human)
Insert Size (bp)1300
Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-EGFP-Cdc42(wt) was a gift from Klaus Hahn (Addgene plasmid # 12599 ; http://n2t.net/addgene:12599 ; RRID:Addgene_12599)
For your References section:Activation of endogenous Cdc42 visualized in living cells. Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM. Science. 2004 Sep 10. 305(5690):1615-9. 10.1126/science.1100367 PubMed 15361624