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An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.
Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72872PMXS-GOT1The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
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72873PMXS-SLC1A3The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
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72874plentiCRISPR-sgGOT1The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).
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72875plentiCRISPR-sgMDH1The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB
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110066Human CRISPR Metabolic Gene Knockout LibraryEncodes gRNAs for targeting metabolic genes in CRISPR screens.
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Recombinant Antibodies from Article