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PMXS-GOT1
(Plasmid #72872)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72872 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs-IRES-blasticidin
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 6882
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GOT1
  • Alt name
    AST1; cCAT; GIG18; cAspAT; ASTQTL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1242
  • Mutation
    sgRNA resistant human GOT1, 7 silent mutations c138t, t141c, c144a, c657t,c660a,c663t,c669t
  • GenBank ID
    NM_002079.2
  • Entrez Gene
    GOT1 (a.k.a. AST, AST1, ASTQTL1, GIG18, SGOT, cAspAT, cCAT)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It is a retroviral plasmid so it may be recommended to be propagated at 30oc but author typically grows it at 37oC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PMXS-GOT1 was a gift from David Sabatini (Addgene plasmid # 72872 ; http://n2t.net/addgene:72872 ; RRID:Addgene_72872)
  • For your References section:

    An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM. Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016. 10.1016/j.cell.2015.07.016 PubMed 26232224