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PMXS-SLC1A3
(Plasmid #72873)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72873 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs-IRES-blasticidin
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 7268
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SLC1A3
  • Alt name
    EA6; EAAT1; GLAST; GLAST1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1629
  • GenBank ID
    NM_004172.4
  • Entrez Gene
    SLC1A3 (a.k.a. EA6, EAAT1, GLAST, GLAST1)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCCGGATCTAGCTAGTTAATTAAGccaccATGACTAAAAGCAATGGAGAAGAGCCC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

It is a retroviral plasmid so it may be recommended to be propagated at 30°C but author typically grows it at 37°C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PMXS-SLC1A3 was a gift from David Sabatini (Addgene plasmid # 72873 ; http://n2t.net/addgene:72873 ; RRID:Addgene_72873)
  • For your References section:

    An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis. Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM. Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016. 10.1016/j.cell.2015.07.016 PubMed 26232224