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AAV-CAGGS-EGFP Citations (41)

Originally described in: Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases.
Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R Nat Biotechnol. 2009 Aug 13. ():.
PubMed Journal

Articles Citing AAV-CAGGS-EGFP

Articles
Oxidase-deficient neutrophils from X-linked chronic granulomatous disease iPS cells: functional correction by zinc finger nuclease-mediated safe harbor targeting. Zou J, Sweeney CL, Chou BK, Choi U, Pan J, Wang H, Dowey SN, Cheng L, Malech HL. Blood. 2011 May 26;117(21):5561-72. doi: 10.1182/blood-2010-12-328161. Epub 2011 Mar 16. PubMed
Myocardial tissue engineering and heart muscle repair. Karikkineth BC, Zimmermann WH. Curr Pharm Biotechnol. 2013;14(1):4-11. PubMed
High-precision robotic microcontact printing (R-muCP) utilizing a vision guided selectively compliant articulated robotic arm. McNulty JD, Klann T, Sha J, Salick M, Knight GT, Turng LS, Ashton RS. Lab Chip. 2014 Jun 7;14(11):1923-30. doi: 10.1039/c3lc51137e. Epub 2014 Apr 24. PubMed
Stable Enhanced Green Fluorescent Protein Expression After Differentiation and Transplantation of Reporter Human Induced Pluripotent Stem Cells Generated by AAVS1 Transcription Activator-Like Effector Nucleases. Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J. Stem Cells Transl Med. 2014 May 15. pii: sctm.2013-0212. PubMed

Associated Plasmids

Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease. Luo Y, Rao M, Zou J. Curr Protoc Stem Cell Biol. 2014 May 16;29:5A.7.1-5A.7.18. doi: 10.1002/9780470151808.sc05a07s29. PubMed
Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system. Zhang Y, Vanoli F, LaRocque JR, Krawczyk PM, Jasin M. Methods. 2014 Sep;69(2):171-8. doi: 10.1016/j.ymeth.2014.05.003. Epub 2014 Jun 12. PubMed
Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Smith C, Gore A, Yan W, Abalde-Atristain L, Li Z, He C, Wang Y, Brodsky RA, Zhang K, Cheng L, Ye Z. Cell Stem Cell. 2014 Jul 3;15(1):12-3. doi: 10.1016/j.stem.2014.06.011. PubMed
Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs. Ranganathan V, Wahlin K, Maruotti J, Zack DJ. Nat Commun. 2014 Aug 8;5:4516. doi: 10.1038/ncomms5516. PubMed

Associated Plasmids

An AAVS1-targeted minigene platform for correction of iPSCs from all five types of chronic granulomatous disease. Merling RK, Sweeney CL, Chu J, Bodansky A, Choi U, Priel DL, Kuhns DB, Wang H, Vasilevsky S, De Ravin SS, Winkler T, Dunbar CE, Zou J, Zarember KA, Gallin JI, Holland SM, Malech HL. Mol Ther. 2015 Jan;23(1):147-57. doi: 10.1038/mt.2014.195. Epub 2014 Oct 7. PubMed
Efficient and allele-specific genome editing of disease loci in human iPSCs. Smith C, Abalde-Atristain L, He C, Brodsky BR, Braunstein EM, Chaudhari P, Jang YY, Cheng L, Ye Z. Mol Ther. 2015 Mar;23(3):570-7. doi: 10.1038/mt.2014.226. Epub 2014 Nov 24. PubMed
Transfection, selection, and colony-picking of human induced pluripotent stem cells TALEN-targeted with a GFP gene into the AAVS1 safe harbor. Cerbini T, Luo Y, Rao MS, Zou J. J Vis Exp. 2015 Feb 1;(96). doi: 10.3791/52504. PubMed
High-content imaging with micropatterned multiwell plates reveals influence of cell geometry and cytoskeleton on chromatin dynamics. Harkness T, McNulty JD, Prestil R, Seymour SK, Klann T, Murrell M, Ashton RS, Saha K. Biotechnol J. 2015 Jun 12. doi: 10.1002/biot.201400756. PubMed
Controlling transcription in human pluripotent stem cells using CRISPR-effectors. Genga RM, Kearns NA, Maehr R. Methods. 2015 Oct 23. pii: S1046-2023(15)30132-8. doi: 10.1016/j.ymeth.2015.10.014. PubMed
Multidimensional Genome-wide Analyses Show Accurate FVIII Integration by ZFN in Primary Human Cells. Sivalingam J, Kenanov D, Han H, Nirmal AJ, Ng WH, Lee SS, Masilamani J, Phan TT, Maurer-Stroh S, Kon OL. Mol Ther. 2016 Mar;24(3):607-19. doi: 10.1038/mt.2015.223. Epub 2015 Dec 22. PubMed
High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. Steyer B, Carlson-Stevermer J, Angenent-Mari N, Khalil A, Harkness T, Saha K. Acta Biomater. 2015 Dec 30. pii: S1742-7061(15)30273-7. doi: 10.1016/j.actbio.2015.12.036. PubMed
Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing in Human Cells. Havlicek S, Shen Y, Alpagu Y, Bruntraeger MB, Zufir NB, Phuah ZY, Fu Z, Dunn NR, Stanton LW. Mol Ther. 2017 Feb 1;25(2):342-355. doi: 10.1016/j.ymthe.2016.11.007. PubMed

Associated Plasmids

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery. Gaj T, Staahl BT, Rodrigues GM, Limsirichai P, Ekman FK, Doudna JA, Schaffer DV. Nucleic Acids Res. 2017 Mar 2. doi: 10.1093/nar/gkx154. PubMed
A platform for functional assessment of large variant libraries in mammalian cells. Matreyek KA, Stephany JJ, Fowler DM. Nucleic Acids Res. 2017 Mar 15. doi: 10.1093/nar/gkx183. PubMed
Characterization of a splice-site mutation in the tumor suppressor gene FLCN associated with renal cancer. Bartram MP, Mishra T, Reintjes N, Fabretti F, Gharbi H, Adam AC, Gobel H, Franke M, Schermer B, Haneder S, Benzing T, Beck BB, Muller RU. BMC Med Genet. 2017 May 12;18(1):53. doi: 10.1186/s12881-017-0416-5. PubMed
Acoustic-transfection for genomic manipulation of single-cells using high frequency ultrasound. Yoon S, Wang P, Peng Q, Wang Y, Shung KK. Sci Rep. 2017 Jul 13;7(1):5275. doi: 10.1038/s41598-017-05722-1. PubMed
Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing. Carlson-Stevermer J, Abdeen AA, Kohlenberg L, Goedland M, Molugu K, Lou M, Saha K. Nat Commun. 2017 Nov 23;8(1):1711. doi: 10.1038/s41467-017-01875-9. PubMed
SN-38 Conjugated Gold Nanoparticles Activated by Ewing Sarcoma Specific mRNAs Exhibit In Vitro and In Vivo Efficacy. Naumann JA, Widen JC, Jonart LA, Ebadi M, Tang J, Gordon DJ, Harki DA, Gordon PM. Bioconjug Chem. 2018 Apr 18;29(4):1111-1118. doi: 10.1021/acs.bioconjchem.7b00774. Epub 2018 Feb 19. PubMed
Anatomical Evidence for a Direct Projection from Purkinje Cells in the Mouse Cerebellar Vermis to Medial Parabrachial Nucleus. Hashimoto M, Yamanaka A, Kato S, Tanifuji M, Kobayashi K, Yaginuma H. Front Neural Circuits. 2018 Feb 7;12:6. doi: 10.3389/fncir.2018.00006. eCollection 2018. PubMed
Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development. Chen Z, Ren X, Xu X, Zhang X, Hui Y, Liu Z, Shi L, Fang Y, Ma L, Liu Y, Terheyden-Keighley D, Liu L, Zhang X. Stem Cell Reports. 2018 Nov 13;11(5):1257-1271. doi: 10.1016/j.stemcr.2018.09.014. Epub 2018 Oct 25. PubMed
Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9. Snijders KE, Cooper JD, Vallier L, Bertero A. Methods Mol Biol. 2019;1961:185-209. doi: 10.1007/978-1-4939-9170-9_12. PubMed
A protein-RNA interaction atlas of the ribosome biogenesis factor AATF. Kaiser RWJ, Ignarski M, Van Nostrand EL, Frese CK, Jain M, Cukoski S, Heinen H, Schaechter M, Seufert L, Bunte K, Frommolt P, Keller P, Helm M, Bohl K, Hohne M, Schermer B, Benzing T, Hopker K, Dieterich C, Yeo GW, Muller RU, Fabretti F. Sci Rep. 2019 Jul 30;9(1):11071. doi: 10.1038/s41598-019-47552-3. PubMed
A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing. Chen G, Abdeen AA, Wang Y, Shahi PK, Robertson S, Xie R, Suzuki M, Pattnaik BR, Saha K, Gong S. Nat Nanotechnol. 2019 Oct;14(10):974-980. doi: 10.1038/s41565-019-0539-2. Epub 2019 Sep 9. PubMed
Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure. Lungova V, Chen X, Wang Z, Kendziorski C, Thibeault SL. Nat Commun. 2019 Sep 24;10(1):4161. doi: 10.1038/s41467-019-12069-w. PubMed
Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics. Molugu K, Harkness T, Carlson-Stevermer J, Prestil R, Piscopo NJ, Seymour SK, Knight GT, Ashton RS, Saha K. Biophys J. 2019 Oct 22. pii: S0006-3495(19)30862-8. doi: 10.1016/j.bpj.2019.10.014. PubMed
Long-term development of human iPSC-derived pyramidal neurons quantified after transplantation into the neonatal mouse cortex. D'Alessio R, Koukouli F, Blanchard S, Catteau J, Rais C, Lemonnier T, Feraud O, Bennaceur-Griscelli A, Groszer M, Maskos U. Dev Biol. 2020 May 1;461(1):86-95. doi: 10.1016/j.ydbio.2020.01.009. Epub 2020 Jan 23. PubMed
Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter. Butterfield KT, McGrath PS, Han CM, Kogut I, Bilousova G. Methods Mol Biol. 2020;2155:11-21. doi: 10.1007/978-1-0716-0655-1_2. PubMed
Standardized Reporter Systems for Purification and Imaging of Human Pluripotent Stem Cell-derived Motor Neurons and Other Cholinergic Cells. Garcia-Diaz A, Efe G, Kabra K, Patel A, Lowry ER, Shneider NA, Corneo B, Wichterle H. Neuroscience. 2020 Jun 30. pii: S0306-4522(20)30404-8. doi: 10.1016/j.neuroscience.2020.06.028. PubMed
Generation of a Transplantable Population of Human iPSC-Derived Retinal Ganglion Cells. Rabesandratana O, Chaffiol A, Mialot A, Slembrouck-Brec A, Joffrois C, Nanteau C, Rodrigues A, Gagliardi G, Reichman S, Sahel JA, Chedotal A, Duebel J, Goureau O, Orieux G. Front Cell Dev Biol. 2020 Oct 27;8:585675. doi: 10.3389/fcell.2020.585675. eCollection 2020. PubMed
Novel fluorescent-based reporter cell line engineered for monitoring homologous recombination events. Bernardi A, Gobelli D, Serna J, Nawrocka P, March-Rossello G, Orduna A, Kozlowski P, Simarro M, de la Fuente MA. PLoS One. 2021 Apr 30;16(4):e0237413. doi: 10.1371/journal.pone.0237413. eCollection 2021. PubMed
Extracellular vesicles released by human retinal pigment epithelium mediate increased polarised secretion of drusen proteins in response to AMD stressors. Flores-Bellver M, Mighty J, Aparicio-Domingo S, Li KV, Shi C, Zhou J, Cobb H, McGrath P, Michelis G, Lenhart P, Bilousova G, Heissel S, Rudy MJ, Coughlan C, Goodspeed AE, Becerra SP, Redenti S, Canto-Soler MV. J Extracell Vesicles. 2021 Nov;10(13):e12165. doi: 10.1002/jev2.12165. PubMed
Enhancement of pacing function by HCN4 overexpression in human pluripotent stem cell-derived cardiomyocytes. Saito Y, Nakamura K, Yoshida M, Sugiyama H, Akagi S, Miyoshi T, Morita H, Ito H. Stem Cell Res Ther. 2022 Apr 1;13(1):141. doi: 10.1186/s13287-022-02818-y. PubMed
Gulo gene locus, a new gene editing locus for mammalian cells. Habu T, Ishikawa H, Kim J. Biotechnol J. 2022 Jul;17(7):e2100493. doi: 10.1002/biot.202100493. Epub 2022 Apr 19. PubMed
Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue. Rodrigues A, Slembrouck-Brec A, Nanteau C, Terray A, Tymoshenko Y, Zagar Y, Reichman S, Xi Z, Sahel JA, Fouquet S, Orieux G, Nandrot EF, Byrne LC, Audo I, Roger JE, Goureau O. NPJ Regen Med. 2022 Aug 16;7(1):39. doi: 10.1038/s41536-022-00235-6. PubMed
In Vivo Delivery of Therapeutic Molecules by Transplantation of Genome-Edited Induced Pluripotent Stem Cells. Nakajima I, Tsukimura T, Ono T, Shiga T, Shitara H, Togawa T, Sakuraba H, Miyaoka Y. Cell Transplant. 2023 Jan-Dec;32:9636897231173734. doi: 10.1177/09636897231173734. PubMed

Associated Plasmids

CRISPRi Gene Modulation and All-Optical Electrophysiology in Post-Differentiated Human iPSC-Cardiomyocytes. Han JL, Heinson YW, Chua CJ, Liu W, Entcheva E. bioRxiv. 2023 May 9:2023.05.07.539756. doi: 10.1101/2023.05.07.539756. Preprint. PubMed
CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes. Han JL, Heinson YW, Chua CJ, Liu W, Entcheva E. Commun Biol. 2023 Dec 7;6(1):1236. doi: 10.1038/s42003-023-05627-y. PubMed

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