pH1v1
(Plasmid
#60244)
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Purpose(Empty Backbone) gRNA expression by the H1 promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Backbone manufacturerNEB
- Backbone size (bp) 2799
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Modifications to backboneH1 promoter and gRNA scaffold was cloned into pUC19:NdeI-XbaI digest. Digest pH1v1 plasmid with AvrII to insert gRNA target sequence by Gibson reaction.
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Vector typeMammalian Expression, CRISPR
- Promoter H1 promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TGTCGGGGCTGGCTTAAC
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH1v1 was a gift from Donald Zack (Addgene plasmid # 60244 ; http://n2t.net/addgene:60244 ; RRID:Addgene_60244) -
For your References section:
Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs. Ranganathan V, Wahlin K, Maruotti J, Zack DJ. Nat Commun. 2014 Aug 8;5:4516. doi: 10.1038/ncomms5516. 10.1038/ncomms5516 PubMed 25105359
Map uploaded by the depositor.
