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pH1v1
(Plasmid #60244)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60244 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    NEB
  • Backbone size (bp) 2799
  • Modifications to backbone
    H1 promoter and gRNA scaffold was cloned into pUC19:NdeI-XbaI digest. Digest pH1v1 plasmid with AvrII to insert gRNA target sequence by Gibson reaction.
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter H1 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH1v1 was a gift from Donald Zack (Addgene plasmid # 60244 ; http://n2t.net/addgene:60244 ; RRID:Addgene_60244)
  • For your References section:

    Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs. Ranganathan V, Wahlin K, Maruotti J, Zack DJ. Nat Commun. 2014 Aug 8;5:4516. doi: 10.1038/ncomms5516. 10.1038/ncomms5516 PubMed 25105359