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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
(Plasmid #61592)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61592 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSaCas9
  • Insert Size (bp)
    3345
  • GenBank ID
    CCK74173.1
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • 3xHA (C terminal on insert)

Resource Information

Depositor Comments

Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA was a gift from Feng Zhang (Addgene plasmid # 61592)
  • For your References section:

    In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature. 2015 Apr 1. doi: 10.1038/nature14299. 10.1038/nature14299 PubMed 25830891