PurposeA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61592||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)3345
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS (C terminal on insert)
- 3xHA (C terminal on insert)
Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA was a gift from Feng Zhang (Addgene plasmid # 61592 ; http://n2t.net/addgene:61592 ; RRID:Addgene_61592)
For your References section:In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature. 2015 Apr 1. doi: 10.1038/nature14299. 10.1038/nature14299 PubMed 25830891