In vivo genome editing using Staphylococcus aureus Cas9.
Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
Nature. 2015 Apr 1. doi: 10.1038/nature14299.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61591 | pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA | A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. |
| 61592 | pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA | A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). |
| 61593 | pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA | A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. |
| 61594 | pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA | A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations. |