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(Plasmid #12243)


Item Catalog # Description Quantity Price (USD)
Plasmid 12243 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 10438
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy


  • Gene/Insert name
    GFP, HSV1-TK
  • Alt name
  • Alt name
    HSV-1 thymidine kinase
  • Mutation
    LoxP in 3' LTR.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV Fwd for EGFP
  • 3′ sequencing primer SP6 primer rev for LoxP
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CMV promoter is upstream of the bicistronic coding cassette with GFP and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab for cloning strategies, protocols, publications, and more. See LentiWeb for discussion on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOX-gfp-iresTK was a gift from Didier Trono (Addgene plasmid # 12243 ; ; RRID:Addgene_12243)
  • For your References section:

    Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D. Mol Ther. 2000 Oct . 2(4):404-14. 10.1006/mthe.2000.0141 PubMed 11020357