|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12238||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9886
Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
MutationCMV promoter is upstream of nls-CRE. LoxP in 3' LTR.
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV forward
- 3′ sequencing primer SP6 for lox site (Common Sequencing Primers)
Please note that the full sequence for this plasmid is approximated and not fully verified. ALTHOUGH THE SEQUENCE OF THIS PLASMID SUGGESTS IT'S FULL SIZE IS 9.9KB, OUR TEST DIGEST SHOWS IT IS ACTUALLY 11.5KB. NONETHELESS, OUR CUSTOMERS HAVE BEEN ABLE TO MAKE FUNCTIONAL VIRAL PARTICLES USING THIS PLASMID.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLOX-CW-CRE was a gift from Didier Trono (Addgene plasmid # 12238 ; http://n2t.net/addgene:12238 ; RRID:Addgene_12238)
For your References section:Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability. Cudre-Mauroux C, Occhiodoro T, Konig S, Salmon P, Bernheim L, Trono D. Hum Gene Ther. 2003 Nov 1. 14(16):1525-33. 10.1089/104303403322495034 PubMed 14577914