|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12240||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9967
Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationCMV promoter upstream of mBmi-1. LoxP in 3' LTR.
Entrez GeneBmi1 (a.k.a. AW546694, Bmi-1, Pcgf4)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV fwd
- 3′ sequencing primer SP6 for LoxP site (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLOX-CWBmi1 was a gift from Didier Trono (Addgene plasmid # 12240 ; http://n2t.net/addgene:12240 ; RRID:Addgene_12240)
For your References section:Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D. Mol Ther. 2000 Oct . 2(4):404-14. 10.1006/mthe.2000.0141 PubMed 11020357