169,069 results

1. AAVS1-TALEN-L

   Plasmid

   #59025

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-L
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-TALEN-R

   Plasmid

   #59026

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-R
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMApra-Ptac

   Plasmid

   #218357

   Purpose

   Empty vector with lacIq-Ptac expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2/8

   Plasmid

   #112864

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap8
   

   Use

   AAV

   Promoter

   Truncated P5

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_PE7-2A-BSD

   Plasmid

   #234073

   Purpose

   For lentiviral expression of PE7 with blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PE7
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF-1α core promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRGFP

   Plasmid

   #26475

   Purpose

   In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

   Depositor

   Maria Jasin

   Article

   Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

   Insert

   DRGFP
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-p65

   Plasmid

   #111190

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

   Insert

   RELA (RELA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mcherry-DN KASH

    Plasmid

    #125553

    Purpose

    mcherry labeled DN-KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAF0033 Ef1a-Pa01-2a-EGFP

    Plasmid

    #193460

    Purpose

    Expression of Pa01 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Tags

    TurboRFP

    Expression

    Mammalian

    Promoter

    13X repeated p21-p53 response element

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/Myc-DNMT1

    Plasmid

    #36939

    Purpose

    Mammalian expression of human DNMT1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

    Insert

    DNMT1 (DNMT1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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