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Plasmid#196705PurposeAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationLys to Val substitution at position 612PromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar9si
Plasmid#222834PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoSNAP
Plasmid#136623PurposePlasmid encoding outer mitochondrial localization SNAP-TagDepositorInsertNTOM20-SNAP-Tag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJA54 HLA-A*03:01
Plasmid#180451PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*03:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis
Plasmid#156543PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPDCD1 (PDCD1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-DCX-TurboID-V5-puro
Plasmid#190742PurposeA lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)DepositorInsertDCX-TurboID-V5 (DCX DCX is human doublecortin; TurboID is engineered BirA from E.coli, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-LaminB1-10
Plasmid#58107PurposeLocalization: Nuclear Envelope, Excitation: 554, Emission: 581DepositorInsertLaminB1 (LMNB1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAquaN1
Plasmid#42888DepositorInsertAquamarine
UseTagsExpressionMammalianMutationT66S and H149GPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLV-mRFP1-Human UTROPHIN_1-261
Plasmid#225052PurposeExpress in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and RFP1 (UTRN Homo Sapiens, Human)
UseLentiviralTagsHuman UTROPHIN 1-261 and mRFP1ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26739-R…PromoterCMV promoter and enhancerAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-Human-UTROPHIN_1-261
Plasmid#222393PurposeExpress in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and EGFP (UTRN Homo Sapiens, Human)
UseLentiviralTagsEGFP and Human UTROPHIN 1-261ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26737-G…PromoterCMV promoter and enhancerAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG106K
Plasmid#191377PurposeBacteroides - Escherichia coli shuttle vector with ErmF and Kan selection markerDepositorTypeEmpty backboneUsePg106TagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10Smt3-gamma-toxin
Plasmid#197000PurposeBacterial expression plasmid for gamma toxin tRNA endonucleaseDepositorInsertGamma toxin
UseTagsHis10-Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTrcAgBIS (CO)
Plasmid#35153DepositorInsertBisabolene Synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpTrcAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-MDA5-Full-length
Plasmid#210826Purposeprotein expression in ecoliDepositorInsertMDA5 (IFIH1) Full Length Protein (IFIH1 Human)
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VP192-T2A-EGFP
Plasmid#69536PurposeEpisomal plasmid encoding dCas9VP192DepositorInsertdCas9-dCas9VP192-EGFP
UseCRISPRTagsEGFPExpressionMutationPromoterCAGAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CJ8421_04975 Rptr2 and CJ8421_04975 mRNA
Plasmid#194210PurposeExpresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2DepositorInsertCJ8421_04975 mRNA and CJ8421_04975 Rptr2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only