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An enhanced CRISPR repressor for targeted mammalian gene regulation.
Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC, Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET, Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church GM
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110820dCas9-KRABConventional dCas9 repressor-dCas9-KRAB
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110821dCas9-KRAB-MeCP2Improved dCas9 repressor-dCas9-KRAB-MeCP2
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110822pB-CAGGS-dCas9-KRABPiggyBac compatible plasmid expressing dCas9-KRAB
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110823pB-CAGGS-dCas9PiggyBac compatible plasmid expressing dCas9
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110824pB-CAGGS-dCas9-KRAB-MeCP2PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
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