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An enhanced CRISPR repressor for targeted mammalian gene regulation.
Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC, Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET, Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church GM
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110820dCas9-KRABConventional dCas9 repressor-dCas9-KRAB
110821dCas9-KRAB-MeCP2Improved dCas9 repressor-dCas9-KRAB-MeCP2
110822pB-CAGGS-dCas9-KRABPiggyBac compatible plasmid expressing dCas9-KRAB
110823pB-CAGGS-dCas9PiggyBac compatible plasmid expressing dCas9
110824pB-CAGGS-dCas9-KRAB-MeCP2PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2