|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||43802||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5385
- Total vector size (bp) 9525
Vector typeYeast Expression, CRISPR
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameHuman Optimized S. pyogenes Cas9
SpeciesH. sapiens (human), S. cerevisiae (budding yeast); S.pyogenes
Insert Size (bp)4140
- Promoter TEF1 promoter
- Cloning method Unknown
- 5′ sequencing primer T3 promoter
- 3′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p414-TEF1p-Cas9-CYC1t was a gift from George Church (Addgene plasmid # 43802 ; http://n2t.net/addgene:43802 ; RRID:Addgene_43802)
For your References section:Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.. Nucleic Acids Res 10.1093/nar/gkt135 PubMed 23460208