PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46920||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6900
- Total vector size (bp) 11054
Vector typeYeast Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4135
- Promoter TDH3
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gGAGCTCactcgGAATTCagtctACTAGT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTDH3-dCas9 was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid # 46920 ; http://n2t.net/addgene:46920 ; RRID:Addgene_46920)
For your References section:CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. 10.1016/j.cell.2013.06.044 PubMed 23849981