p414-TEF1p-Cas9-CYC1t Citations (64)
Originally described in: Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM. Nucleic Acids Res PubMed Journal
Articles Citing p414-TEF1p-Cas9-CYC1t
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Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS.
Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
PubMed
Associated Plasmids |
| Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae. Jakociunas T, Bonde I, Herrgard M, Harrison SJ, Kristensen M, Pedersen LE, Jensen MK, Keasling JD. Metab Eng. 2015 Mar;28:213-22. doi: 10.1016/j.ymben.2015.01.008. Epub 2015 Jan 28. PubMed |
| Evolution of gene network activity by tuning the strength of negative-feedback regulation. Peng W, Liu P, Xue Y, Acar M. Nat Commun. 2015 Feb 11;6:6226. doi: 10.1038/ncomms7226. PubMed |
| CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae. Jakociunas T, Rajkumar AS, Zhang J, Arsovska D, Rodriguez A, Jendresen CB, Skjodt ML, Nielsen AT, Borodina I, Jensen MK, Keasling JD. ACS Synth Biol. 2015 Nov 20;4(11):1226-1234. Epub 2015 Mar 26. PubMed |
| Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus. Fuller KK, Chen S, Loros JJ, Dunlap JC. Eukaryot Cell. 2015 Nov;14(11):1073-80. doi: 10.1128/EC.00107-15. Epub 2015 Aug 28. PubMed |
| Safeguarding CRISPR-Cas9 gene drives in yeast. DiCarlo JE, Chavez A, Dietz SL, Esvelt KM, Church GM. Nat Biotechnol. 2015 Dec;33(12):1250-1255. doi: 10.1038/nbt.3412. Epub 2015 Nov 16. PubMed |
| In-Yeast Engineering of a Bacterial Genome Using CRISPR/Cas9. Tsarmpopoulos I, Gourgues G, Blanchard A, Vashee S, Jores J, Lartigue C, Sirand-Pugnet P. ACS Synth Biol. 2016 Jan 15;5(1):104-9. doi: 10.1021/acssynbio.5b00196. Epub 2015 Dec 7. PubMed |
| CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae. Sasano Y, Nagasawa K, Kaboli S, Sugiyama M, Harashima S. Sci Rep. 2016 Aug 17;6:30278. doi: 10.1038/srep30278. PubMed |
| CAR1 deletion by CRISPR/Cas9 reduces formation of ethyl carbamate from ethanol fermentation by Saccharomyces cerevisiae. Chin YW, Kang WK, Jang HW, Turner TL, Kim HJ. J Ind Microbiol Biotechnol. 2016 Nov;43(11):1517-1525. Epub 2016 Aug 29. PubMed |
Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.
Arras SD, Chua SM, Wizrah MS, Faint JA, Yap AS, Fraser JA.
PLoS One. 2016 Oct 6;11(10):e0164322. doi: 10.1371/journal.pone.0164322. eCollection 2016.
PubMed
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| Towards the exploitation of glycerol's high reducing power in Saccharomyces cerevisiae-based bioprocesses. Klein M, Carrillo M, Xiberras J, Islam ZU, Swinnen S, Nevoigt E. Metab Eng. 2016 Nov;38:464-472. doi: 10.1016/j.ymben.2016.10.008. Epub 2016 Oct 14. PubMed |
| Cloning and Functional Characterization of Cycloartenol Synthase from the Red Seaweed Laurencia dendroidea. Calegario G, Pollier J, Arendt P, de Oliveira LS, Thompson C, Soares AR, Pereira RC, Goossens A, Thompson FL. PLoS One. 2016 Nov 10;11(11):e0165954. doi: 10.1371/journal.pone.0165954. eCollection 2016. PubMed |
| Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism. Sonawane PD, Pollier J, Panda S, Szymanski J, Massalha H, Yona M, Unger T, Malitsky S, Arendt P, Pauwels L, Almekias-Siegl E, Rogachev I, Meir S, Cardenas PD, Masri A, Petrikov M, Schaller H, Schaffer AA, Kamble A, Giri AP, Goossens A, Aharoni A. Nat Plants. 2016 Dec 22;3:16205. doi: 10.1038/nplants.2016.205. PubMed |
| A Precise Genome Editing Method Reveals Insights into the Activity of Eukaryotic Promoters. Elison GL, Song R, Acar M. Cell Rep. 2017 Jan 3;18(1):275-286. doi: 10.1016/j.celrep.2016.12.014. PubMed |
| The ancient CYP716 family is a major contributor to the diversification of eudicot triterpenoid biosynthesis. Miettinen K, Pollier J, Buyst D, Arendt P, Csuk R, Sommerwerk S, Moses T, Mertens J, Sonawane PD, Pauwels L, Aharoni A, Martins J, Nelson DR, Goossens A. Nat Commun. 2017 Feb 6;8:14153. doi: 10.1038/ncomms14153. PubMed |
| SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae. Vanegas KG, Lehka BJ, Mortensen UH. Microb Cell Fact. 2017 Feb 8;16(1):25. doi: 10.1186/s12934-017-0632-x. PubMed |
| Hsp104 disaggregase at normal levels cures many [PSI(+)] prion variants in a process promoted by Sti1p, Hsp90, and Sis1p. Gorkovskiy A, Reidy M, Masison DC, Wickner RB. Proc Natl Acad Sci U S A. 2017 May 23;114(21):E4193-E4202. doi: 10.1073/pnas.1704016114. Epub 2017 May 8. PubMed |
CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus.
Lobs AK, Engel R, Schwartz C, Flores A, Wheeldon I.
Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017.
PubMed
Associated Plasmids |
| Efficient genome editing by CRISPR/Cas9 with a tRNA-sgRNA fusion in the methylotrophic yeast Ogataea polymorpha. Numamoto M, Maekawa H, Kaneko Y. J Biosci Bioeng. 2017 Jun 27. pii: S1389-1723(17)30214-1. doi: 10.1016/j.jbiosc.2017.06.001. PubMed |
| FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Swiat MA, Dashko S, den Ridder M, Wijsman M, van der Oost J, Daran JM, Daran-Lapujade P. Nucleic Acids Res. 2017 Dec 1;45(21):12585-12598. doi: 10.1093/nar/gkx1007. PubMed |
| Biochemical studies of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare and their roles in lignocellulose degradation. Liu B, Olson A, Wu M, Broberg A, Sandgren M. PLoS One. 2017 Dec 11;12(12):e0189479. doi: 10.1371/journal.pone.0189479. eCollection 2017. PubMed |
| The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae. Bracher JM, Verhoeven MD, Wisselink HW, Crimi B, Nijland JG, Driessen AJM, Klaassen P, van Maris AJA, Daran JG, Pronk JT. Biotechnol Biofuels. 2018 Mar 13;11:63. doi: 10.1186/s13068-018-1047-6. eCollection 2018. PubMed |
| Altered access to beneficial mutations slows adaptation and biases fixed mutations in diploids. Marad DA, Buskirk SW, Lang GI. Nat Ecol Evol. 2018 May;2(5):882-889. doi: 10.1038/s41559-018-0503-9. Epub 2018 Mar 26. PubMed |
| Locus specific engineering of tandem DNA repeats in the genome of Saccharomyces cerevisiae using CRISPR/Cas9 and overlapping oligonucleotides. Lancrey A, Joubert A, Boule JB. Sci Rep. 2018 May 8;8(1):7127. doi: 10.1038/s41598-018-25508-3. PubMed |
| A protocol for introduction of multiple genetic modifications in Saccharomyces cerevisiae using CRISPR/Cas9. Mans R, Wijsman M, Daran-Lapujade P, Daran JM. FEMS Yeast Res. 2018 Nov 1;18(7). pii: 5026622. doi: 10.1093/femsyr/foy063. PubMed |
| Engineering de novo anthocyanin production in Saccharomyces cerevisiae. Levisson M, Patinios C, Hein S, de Groot PA, Daran JM, Hall RD, Martens S, Beekwilder J. Microb Cell Fact. 2018 Jul 3;17(1):103. doi: 10.1186/s12934-018-0951-6. PubMed |
| Co-expression of squalene epoxidases with triterpene cyclases boosts production of triterpenoids in plants and yeast. Dong L, Pollier J, Bassard JE, Ntallas G, Almeida A, Lazaridi E, Khakimov B, Arendt P, de Oliveira LS, Lota F, Goossens A, Michoux F, Bak S. Metab Eng. 2018 Sep;49:1-12. doi: 10.1016/j.ymben.2018.07.002. Epub 2018 Jul 23. PubMed |
Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing.
Sharon E, Chen SA, Khosla NM, Smith JD, Pritchard JK, Fraser HB.
Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. doi: 10.1016/j.cell.2018.08.057.
PubMed
Associated Plasmids |
| Genomic and phenotypic characterization of a refactored xylose-utilizing Saccharomyces cerevisiae strain for lignocellulosic biofuel production. Tran Nguyen Hoang P, Ko JK, Gong G, Um Y, Lee SM. Biotechnol Biofuels. 2018 Sep 29;11:268. doi: 10.1186/s13068-018-1269-7. eCollection 2018. PubMed |
| Heat-stress triggers MAPK crosstalk to turn on the hyperosmotic response pathway. Dunayevich P, Baltanas R, Clemente JA, Couto A, Sapochnik D, Vasen G, Colman-Lerner A. Sci Rep. 2018 Oct 11;8(1):15168. doi: 10.1038/s41598-018-33203-6. PubMed |
| Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter. Elison GL, Xue Y, Song R, Acar M. Cell Rep. 2018 Oct 16;25(3):737-748.e4. doi: 10.1016/j.celrep.2018.09.050. PubMed |
| Contribution of Eat1 and Other Alcohol Acyltransferases to Ester Production in Saccharomyces cerevisiae. Kruis AJ, Gallone B, Jonker T, Mars AE, van Rijswijck IMH, Wolkers-Rooijackers JCM, Smid EJ, Steensels J, Verstrepen KJ, Kengen SWM, van der Oost J, Weusthuis RA. Front Microbiol. 2018 Dec 21;9:3202. doi: 10.3389/fmicb.2018.03202. eCollection 2018. PubMed |
| Proteomic characterization of the arsenic response locus in S. cerevisiae. West KL, Byrum SD, Mackintosh SG, Edmondson RD, Taverna SD, Tackett AJ. Epigenetics. 2019 Feb;14(2):130-145. doi: 10.1080/15592294.2019.1580110. Epub 2019 Mar 1. PubMed |
| gRNA-transient expression system for simplified gRNA delivery in CRISPR/Cas9 genome editing. Easmin F, Hassan N, Sasano Y, Ekino K, Taguchi H, Harashima S. J Biosci Bioeng. 2019 Sep;128(3):373-378. doi: 10.1016/j.jbiosc.2019.02.009. Epub 2019 Apr 19. PubMed |
| Production of 14alpha-hydroxysteroids by a recombinant Saccharomyces cerevisiae biocatalyst expressing of a fungal steroid 14alpha-hydroxylation system. Chen J, Tang J, Xi Y, Dai Z, Bi C, Chen X, Fan F, Zhang X. Appl Microbiol Biotechnol. 2019 Oct;103(20):8363-8374. doi: 10.1007/s00253-019-10076-x. Epub 2019 Aug 14. PubMed |
| Deletion of glycerol-3-phosphate dehydrogenase genes improved 2,3-butanediol production by reducing glycerol production in pyruvate decarboxylase-deficient Saccharomyces cerevisiae. Kim JW, Lee YG, Kim SJ, Jin YS, Seo JH. J Biotechnol. 2019 Oct 10;304:31-37. doi: 10.1016/j.jbiotec.2019.08.009. Epub 2019 Aug 14. PubMed |
| CRISPR-PCD and CRISPR-PCRep: Two novel technologies for simultaneous multiple segmental chromosomal deletion/replacement in Saccharomyces cerevisiae. Easmin F, Sasano Y, Kimura S, Hassan N, Ekino K, Taguchi H, Harashima S. J Biosci Bioeng. 2020 Feb;129(2):129-139. doi: 10.1016/j.jbiosc.2019.08.004. Epub 2019 Oct 1. PubMed |
| Identification of Absidia orchidis steroid 11beta-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Chen J, Fan F, Qu G, Tang J, Xi Y, Bi C, Sun Z, Zhang X. Metab Eng. 2020 Jan;57:31-42. doi: 10.1016/j.ymben.2019.10.006. Epub 2019 Oct 24. PubMed |
| Improved simultaneous co-fermentation of glucose and xylose by Saccharomyces cerevisiae for efficient lignocellulosic biorefinery. Hoang Nguyen Tran P, Ko JK, Gong G, Um Y, Lee SM. Biotechnol Biofuels. 2020 Jan 22;13:12. doi: 10.1186/s13068-019-1641-2. eCollection 2020. PubMed |
| Engineering chimeric diterpene synthases and isoprenoid biosynthetic pathways enables high-level production of miltiradiene in yeast. Hu T, Zhou J, Tong Y, Su P, Li X, Liu Y, Liu N, Wu X, Zhang Y, Wang J, Gao L, Tu L, Lu Y, Jiang Z, Zhou YJ, Gao W, Huang L. Metab Eng. 2020 Jul;60:87-96. doi: 10.1016/j.ymben.2020.03.011. Epub 2020 Apr 5. PubMed |
| Engineering Saccharomyces cerevisiae for production of the valuable monoterpene d-limonene during Chinese Baijiu fermentation. Hu Z, Lin L, Li H, Li P, Weng Y, Zhang C, Yu A, Xiao D. J Ind Microbiol Biotechnol. 2020 Jul;47(6-7):511-523. doi: 10.1007/s10295-020-02284-6. Epub 2020 Jun 3. PubMed |
| The evolution of coexistence from competition in experimental co-cultures of Escherichia coli and Saccharomyces cerevisiae. Barber JN, Sezmis AL, Woods LC, Anderson TD, Voss JM, McDonald MJ. ISME J. 2021 Mar;15(3):746-761. doi: 10.1038/s41396-020-00810-z. Epub 2020 Oct 22. PubMed |
| Improve the production of D-limonene by regulating the mevalonate pathway of Saccharomyces cerevisiae during alcoholic beverage fermentation. Hu Z, Li H, Weng Y, Li P, Zhang C, Xiao D. J Ind Microbiol Biotechnol. 2020 Dec;47(12):1083-1097. doi: 10.1007/s10295-020-02329-w. Epub 2020 Nov 15. PubMed |
| High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae. Liu M, Lin YC, Guo JJ, Du MM, Tao X, Gao B, Zhao M, Ma Y, Wang FQ, Wei DZ. ACS Synth Biol. 2021 Jan 15;10(1):158-172. doi: 10.1021/acssynbio.0c00521. Epub 2021 Jan 4. PubMed |
| Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy. Manger S, Ermel UH, Frangakis AS. Commun Biol. 2021 Feb 19;4(1):234. doi: 10.1038/s42003-021-01752-8. PubMed |
| Engineered yeast genomes accurately assembled from pure and mixed samples. Collins JH, Keating KW, Jones TR, Balaji S, Marsan CB, Como M, Newlon ZJ, Mitchell T, Bartley B, Adler A, Roehner N, Young EM. Nat Commun. 2021 Mar 5;12(1):1485. doi: 10.1038/s41467-021-21656-9. PubMed |
A synthetic RNA-mediated evolution system in yeast.
Jensen ED, Laloux M, Lehka BJ, Pedersen LE, Jakociunas T, Jensen MK, Keasling JD.
Nucleic Acids Res. 2021 Sep 7;49(15):e88. doi: 10.1093/nar/gkab472.
PubMed
Associated Plasmids |
| Fine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants. Xu L, Liu P, Dai Z, Fan F, Zhang X. Microb Cell Fact. 2021 Jul 28;20(1):148. doi: 10.1186/s12934-021-01641-z. PubMed |
| PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System. Hassan N, Easmin F, Ekino K, Harashima S. Bio Protoc. 2021 Jul 5;11(13):e4082. doi: 10.21769/BioProtoc.4082. eCollection 2021 Jul 5. PubMed |
| Metabolic engineering of Saccharomyces cerevisiae for gram-scale diosgenin production. Xu L, Wang D, Chen J, Li B, Li Q, Liu P, Qin Y, Dai Z, Fan F, Zhang X. Metab Eng. 2022 Mar;70:115-128. doi: 10.1016/j.ymben.2022.01.013. Epub 2022 Jan 24. PubMed |
| Nucleosome Positioning on Large Tandem DNA Repeats of the '601' Sequence Engineered in Saccharomyces cerevisiae. Lancrey A, Joubert A, Duvernois-Berthet E, Routhier E, Raj S, Thierry A, Sigarteu M, Ponger L, Croquette V, Mozziconacci J, Boule JB. J Mol Biol. 2022 Apr 15;434(7):167497. doi: 10.1016/j.jmb.2022.167497. Epub 2022 Feb 18. PubMed |
| LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress. Lazari LC, Wolf IR, Schnepper AP, Valente GT. PLoS Comput Biol. 2022 May 19;18(5):e1010081. doi: 10.1371/journal.pcbi.1010081. eCollection 2022 May. PubMed |
| Carbon dioxide fixation via production of succinic acid from glycerol in engineered Saccharomyces cerevisiae. Malubhoy Z, Bahia FM, de Valk SC, de Hulster E, Rendulic T, Ortiz JPR, Xiberras J, Klein M, Mans R, Nevoigt E. Microb Cell Fact. 2022 May 28;21(1):102. doi: 10.1186/s12934-022-01817-1. PubMed |
| Polygenic Analysis of Tolerance to Carbon Dioxide Inhibition of Isoamyl Acetate "Banana" Flavor Production in Yeast Reveals MDS3 as Major Causative Gene. Souffriau B, Holt S, Hagman A, De Graeve S, Malcorps P, Foulquie-Moreno MR, Thevelein JM. Appl Environ Microbiol. 2022 Sep 22;88(18):e0081422. doi: 10.1128/aem.00814-22. Epub 2022 Sep 8. PubMed |
| Improving glycerol utilization during high-temperature xylitol production with Kluyveromyces marxianus using a transient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system. Ren L, Liu Y, Xia Y, Huang Y, Liu Y, Wang Y, Li P, Chang K, Xu D, Li F, Zhang B. Bioresour Technol. 2022 Dec;365:128179. doi: 10.1016/j.biortech.2022.128179. Epub 2022 Oct 22. PubMed |
An enChIP system for the analysis of genome functions in budding yeast.
Fujii H, Fujita T.
Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 10.1093/biomethods/bpac025. eCollection 2022.
PubMed
Associated Plasmids |
Genome-wide base editor screen identifies regulators of protein abundance in yeast.
Schubert OT, Bloom JS, Sadhu MJ, Kruglyak L.
Elife. 2022 Nov 3;11. pii: 79525. doi: 10.7554/eLife.79525.
PubMed
Associated Plasmids |
| Construction and evaluation of gRNA arrays for multiplex CRISPR-Cas9. Zun G, Dobersek K, Petrovic U. Yeast. 2023 Jan;40(1):32-41. doi: 10.1002/yea.3833. Epub 2023 Jan 6. PubMed |
| Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae. Wolf IR, Marques LF, de Almeida LF, Lazari LC, de Moraes LN, Cardoso LH, Alves CCO, Nakajima RT, Schnepper AP, Golim MA, Cataldi TR, Nijland JG, Pinto CM, Fioretto MN, Almeida RO, Driessen AJM, Simoes RP, Labate MV, Grotto RMT, Labate CA, Fernandes Junior A, Justulin LA, Coan RLB, Ramos E, Furtado FB, Martins C, Valente GT. Int J Mol Sci. 2023 Mar 15;24(6):5646. doi: 10.3390/ijms24065646. PubMed |
| PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in Saccharomyces cerevisiae. Machens F, Ran G, Ruehmkorff C, Meyer Auf der Heyde J, Mueller-Roeber B, Hochrein L. ACS Synth Biol. 2023 Apr 21;12(4):1046-1057. doi: 10.1021/acssynbio.2c00517. Epub 2023 Apr 4. PubMed |
CREEPY: CRISPR-mediated editing of synthetic episomes in yeast.
Zhao Y, Coelho C, Lauer S, Majewski M, Laurent JM, Brosh R, Boeke JD.
Nucleic Acids Res. 2023 Jun 16:gkad491. doi: 10.1093/nar/gkad491.
PubMed
Associated Plasmids |
| Comparative analyses of disease-linked missense mutations in the RNA exosome modeled in budding yeast reveal distinct functional consequences in translation. Sterrett MC, Cureton LA, Cohen LN, van Hoof A, Khoshnevis S, Fasken MB, Corbett AH, Ghalei H. bioRxiv [Preprint]. 2023 Oct 19:2023.10.18.562946. doi: 10.1101/2023.10.18.562946. PubMed |
| A Biallelic Variant of the RNA Exosome Gene EXOSC4 Causes Translational Defects Associated with a Neurodevelopmental Disorder. Fasken MB, Leung SW, Cureton LA, Al-Awadi M, Al-Kindy A, Khoshnevis S, Ghalei H, Al-Maawali A, Corbett AH. medRxiv [Preprint]. 2023 Oct 28:2023.10.24.23297197. doi: 10.1101/2023.10.24.23297197. PubMed |
| A toolbox for manipulating the genome of the major goat pathogen, Mycoplasma capricolum subsp. capripneumoniae. Gourgues G, Manso-Silvan L, Chamberland C, Sirand-Pugnet P, Thiaucourt F, Blanchard A, Baby V, Lartigue C. Microbiology (Reading). 2024 Jan;170(1):001423. doi: 10.1099/mic.0.001423. PubMed |
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