Purpose(Empty Backbone) Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||128354||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBlueScript II SK(-)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHygromycin (HYG)
- Promoter ACT1
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSDMA65 was a gift from James Fraser (Addgene plasmid # 128354 ; http://n2t.net/addgene:128354 ; RRID:Addgene_128354)