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James Fraser Lab: Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.
Arras SD, Chua SM, Wizrah MS, Faint JA, Yap AS, Fraser JA
PLoS One. 2016 Oct 6;11(10):e0164322. doi: 10.1371/journal.pone.0164322. eCollection 2016.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
128354pSDMA65Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.
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128355pSDMA66gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
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128356pSDMA67gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
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