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(Plasmid #62190)


Item Catalog # Description Quantity Price (USD)
Plasmid 62190 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5385
  • Total vector size (bp) 9596
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
  • Mutation
    human codon-optimized, D10A, H840A
  • Promoter TEF1 promoter
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    George Church (Addgene plasmid 43802)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is compatible with gRNA expression plasmids for budding yeast, such as Addgene Plasmid #43803 ( ).

For more information on Fujii Lab CRISPR Plasmids please refer to:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFLAG-dCas9/pTEF1p-CYC1t was a gift from Hodaka Fujii (Addgene plasmid # 62190 ; ; RRID:Addgene_62190)
  • For your References section:

    An enChIP system for the analysis of genome functions in budding yeast. Fujii H, Fujita T. Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 10.1093/biomethods/bpac025. eCollection 2022. 10.1093/biomethods/bpac025 PubMed 36325175